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Autophagy inhibition sensitizes cisplatin cytotoxicity in human gastric cancer cell line SGC7901.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Hui-Qing Fang, Nian Lu, Shan Liao, Yu-Qian |
| Copyright Year | 2013 |
| Abstract | We aimed to investigate the mechanism and effects of autophagy on cisplatin (DDP)-induced apoptosis in human gastric cancer cell line SGC7901. After SGC7901 cells were treated with DDP and/or chloroquine, cell proliferation was measured using MTT assay; cell apoptosis was determined by flow cytometry; autophagy and apotosis-related proteins expression were detected by Western blot; and quantitative analysis of autophagy after monodansylcadaverine (MDC) staining was performed using fluorescence microscopy. We found after treatment with 5 mg/L DDP for 24 h, the rates of cell apoptosis were (21.07±2.12)%. Autophagy, characterized by an increase in the number of autophagic vesicles and the level of LC3-II protein was observed in cells treated with DDP. After inhibition of autophagy by chloroquine, the rates of cell apoptosis were increased to (30.16±3.54)%, and the level of Caspase-3 and P53 protein were increased, and Bcl-2 protein was decreased. Therefore, autophagy protects human gastric cancer cell line SGC7901 against DDP-induced apoptosis, inhibition of autophagy can promote apoptosis, and combination therapy with DDP and chloroquine may be a promising therapeutic strategy for gastric cancer. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ocean.kisti.re.kr/downfile/volume/apocp/POCPA9/2013/v14n8/POCPA9_2013_v14n8_4685.pdf |
| PubMed reference number | 24083726v1 |
| Volume Number | 14 |
| Issue Number | 8 |
| Journal | Asian Pacific journal of cancer prevention : APJCP |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Apoptosis Autophagy Cell Proliferation Chloroquine Cisplatin Cultured Cell Line Flow Cytometry Fluorescence Microtubule-Associated Proteins 1A/1B Light Chain 3-II Non-Small Cell Lung Carcinoma Staining method Stomach Carcinoma Vesicle (morphologic abnormality) Western Blot cancer cell caspase-3 monodansylcadaverine monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
