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Anti-proliferation effects of trifolirhizin on MKN45 cells and possible mechanism.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lu, Xin Gang Ma, Jianxia Qiu, Hongfu Yang, Liu Cao, Lei Shen, Jie |
| Copyright Year | 2016 |
| Abstract | Trifolirhizin is a compound isolated from Sophora flavescens. It has been shown to exert cytotoxicity on several cancer cell lines. However, the underlying mechanism remains unknown. MKN45 cells were used as a research model. We assessed the cytotoxicity of trifolirhizin to MKN45 by MTT. Hoechst staining and TUNEL method were used to demonstrate apoptosis. Flow cytometry was used to determine cell cycle and ratio of apoptosis. Caspase activity assay was used to examine the activation of caspase cascade pathways. Western blotting was used to explore the protein levels. Consistently, trifolirhizin inhibited MKN45 xenograft tumor growth in vivo. Trifolirhizin caused a significantly decreased proliferation of MKN45 cells in a time- and dose-dependent manner, with IC50 values of 33.27±2.06 µg/ml at 48 h. Western blot assay manifested that trifolirhizin activated the EGFR-MAPK signaling pathways. This study indicated that trifolirhizin may be a therapeutic application in human gastric cancer therapy. |
| Starting Page | 2785 |
| Ending Page | 2792 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.spandidos-publications.com/or/36/5/2785/download |
| PubMed reference number | 27666116v1 |
| Alternate Webpage(s) | https://doi.org/10.3892/or.2016.5125 |
| DOI | 10.3892/or.2016.5125 |
| Journal | Oncology reports |
| Volume Number | 36 |
| Issue Number | 5 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Apoptosis Cascade Device Component Cell Cycle Cultured Cell Line Flow Cytometry In Situ Nick-End Labeling Inhibitory Concentration 50 Neoplasms Sophora flavescens root extract Staining method Stomach Carcinoma Western Blot Western Blotting Xenograft type of graft cancer cell monooxyethylene trimethylolpropane tristearate trifolirhizin |
| Content Type | Text |
| Resource Type | Article |
