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Autophagy inhibition promotes gambogic acid-induced suppression of growth and apoptosis in glioblastoma cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Luo, Guo-Xuan Cai, Jun Lin, Jessica Luo, Wei-Shi Luo, Heng-Shan Jiang, Yu-Yang Zhang, Yin Shuai |
| Copyright Year | 2012 |
| Abstract | OBJECTIVE To investigate the effects of gambogic acid (GA) on the growth of human malignant glioma cells. METHODS U251MG and U87MG human glioma cell lines were treated with GA and growth and proliferation were investigated by MTT and colony formation assays. Cell apoptosis was analyzed by annexin V FITC/PI flow cytometry, mitochondrial membrane potential assays and DAPI nuclear staining. Monodansylcadaverine (MDC) staining and GFP-LC3 localisation were used to detect autophagy. Western blotting was used to investigate the molecular changes that occurred in the course of GA treatment. RESULTS GA treatment significantly suppressed cell proliferation and colony formation, induced apoptosis in U251 and U87MG glioblastoma cells in a time- and dose-dependent manner. GA treatment also lead to the accumulation of monodansylcadaverine (MDC) in autophagic vacuoles, upregulated expressions of Atg5, Beclin 1 and LC3-II, and the increase of punctate fluorescent signals in glioblastoma cells pre-transfected with GFP-tagged LC3 plasmid. After the combination treatment of autophagy inhitors and GA, GA mediated growth inhibition and apoptotic cell death was further potentiated. CONCLUSION Our results suggested that autophagic responses play roles as a self-protective mechanism in GA-treated glioblastoma cells, and autophagy inhibition could be a novel adjunctive strategy for enhancing chemotherapeutic effect of GA as an anti-malignant glioma agent. |
| Starting Page | 55 |
| Ending Page | 64 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://journal.waocp.org/article_27216_d7bcba8ea712415da5a4fe544397b7de.pdf |
| Alternate Webpage(s) | http://ocean.kisti.re.kr/downfile/volume/apocp/POCPA9/2012/v13n12/POCPA9_2012_v13n12_6211.pdf |
| PubMed reference number | 23464433v1 |
| Volume Number | 13 |
| Issue Number | 12 |
| Journal | Asian Pacific journal of cancer prevention : APJCP |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Annexins Apoptosis Autophagosome Autophagy Cell Death Cell Proliferation Cessation of life Cultured Cell Line DAPI Flow Cytometry Fluorescein-5-isothiocyanate Glioblastoma Green Fluorescent Proteins Malignant Glioma Membrane Potentials Microtubule-Associated Proteins 1A/1B Light Chain 3-II Mitochondrial Membranes Role Playing DIsorder Staining method Tracer U251 Vacuole Western Blotting establishment and maintenance of localization gambogic acid monodansylcadaverine monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
