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Antiinflammatory properties of inducible nitric oxide synthase in acute hyperoxic lung injury.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kobayashi, Honami Hataishi, Ryuji Mitsufuji, Hisashi Tanaka, Mitsuru Jacobson, Margaretha Tomita, Takeshi Zapol, Warren M. Jones, Rosemary C. |
| Copyright Year | 2001 |
| Abstract | The objective of this study was to determine whether endogenous nitric oxide (NO), specifically the inducible NO synthase isoform (iNOS: NOS II), reduces or amplifies lung injury in mice breathing at a high oxygen tension. Previous studies have shown that exogenous (inhaled) NO protects against hyperoxia-induced lung injury, and that endogenous NO derived from iNOS inhibits leukocyte recruitment and protects against lung injury induced by lipopolysaccharide. In the present study, hyperoxia (> 98% O(2) for 72 h) induced acute lung injury in both wild-type and iNOS-deficient mice as determined by elevated albumin and lactate dehydrogenase levels in bronchoalveolar lavage fluid (BALF) and by increased extravascular lung water. Lung injury was greater in iNOS-deficient mice than in wild-type mice and was associated with an increased number of polymorphonuclear leukocytes in BALF. iNOS messenger RNA expression levels increased in the lungs of wild-type hyperoxic mice. Nitrotyrosine, a marker of reactive NO species, was expressed in both wild-type and iNOS-deficient mice in hyperoxia, indicating an iNOS-independent pathway for protein nitration. We conclude that iNOS is capable of reducing pulmonary leukocyte accumulation and lung injury. The data indicate that iNOS induction serves as a protective mechanism to minimize the effects of acute exposure to hyperoxia. |
| File Format | PDF HTM / HTML |
| DOI | 10.1165/ajrcmb.24.4.4218 |
| PubMed reference number | 11306431 |
| Journal | Medline |
| Volume Number | 24 |
| Issue Number | 4 |
| Alternate Webpage(s) | http://intl-ajrcmb.atsjournals.org/content/24/4/390.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1165/ajrcmb.24.4.4218 |
| Journal | American journal of respiratory cell and molecular biology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
