Loading...
Please wait, while we are loading the content...
Similar Documents
Universidade Do Extremo Sul Catarinense - Unesc Unidade Acadêmica Humanas Ciências Educação Programa De Pós-graduação Em Ciências Ambientais Mestrado Em Ciências Ambientais
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rodrigues, Vanessa Torres, Nicolau |
| Copyright Year | 2014 |
| Abstract | The medicinal plants always were utilized by population, in the past they were the main therapeutic resource for treatment diseases. Among these therapeutic resources find the medicinal plant Calea uniflora Less., native by region south find also south central of Brazil, Argentina, Uruguay and Paraguay. This plant is utilized populary to process inflammatory and hematomas. From these informations and due the few studies with C. uniflora, emerged the interest of investigate possible compounds chemical, cytotoxic effects, antinociceptive effects, and antiinflammatory. To the phytochemistry evaluation were analyzed the crude extract of plant by HPLC. To the evaluation about activity cytotoxic, were utilized the MTT test, in vitro, in order to verify as extract and fractions de C. uniflora cause possible effects cytotoxic. To evaluation of antinociceptive activity and anti-inflammatory were utilized models in vivo. This animals models were based in chemical stimuli (writhing induced by acetic acid and formalin test) and thermal stimuli (hot plate), while the motor incoordination was analized by rota rod. To evaluate activity anti-inflammatory was used the model paw edema induced by carrageenan. After the performing the tests, verified in the analyses phytochemistry the presence the flavonoid and alkaloid. In the test cytotoxic in vitro, the crude extract and fractions of ethyl acetate and butanol produce IC50 greater that 58 μg/ml to lineage HaCaT and 48 μg/ml to lineage B-16, thus this values not present a cytotoxic effects. However fraction dichloromethane produced IC50 18 μg/ml, showed significant inhibition when compared to controls vincristine and doxorubicin. In relation of antinociceptive activity, the models presented results significant that correspond to chemical and thermal stimuli in the doses of 100 and 300 mg/kg of the crude extract, when compared with the control groups. The rota rod model showed satisfactory results, since the extract did not cause motor incoordination and sedation. According this results, can be related the flavonoids and alkaloids with pharmacological activities reported by agents of “pastoral da saúde” with the antinociceptive and cytotoxic activities. However further studies are needed to determine the action of the activities described and relate to the chemical compounds present in the plant. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://repositorio.unesc.net/bitstream/1/2766/1/Vanessa%20Rodrigues%20Nicolau%20Torres.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
