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Long-term depression induced by postsynaptic group II metabotropic glutamate receptors linked to phospholipase C and intracellular calcium rises in rat prefrontal cortex.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Otani, Satoru Takita, Masatoshi Crépel, Françis |
| Copyright Year | 2002 |
| Abstract | We have previously shown (Otani et al., 1999b) that bath application of (2S,2'R,3'R)-2-(2',3'-dicarboxycyclopropyl)glycine (DCG IV), the agonist of group II metabotropic glutamate receptors (mGluRs), induces postsynaptic Ca2+-dependent long-term depression (LTD) of layer I-II to layer V pyramidal neuron glutamatergic synapses of rat medial prefrontal cortex. In the present study, we examined detailed mechanisms of this DCG IV-induced LTD. First, the group II mGluR antagonist (RS)-alpha-methylserine-O-phosphate monophenyl ester blocked DCG IV-induced LTD, and another group II agonist (2S,3S,4S)-CCG/(2S,1'S,2'S)-2-(carboxycyclopropyl)glycine-induced LTD, suggesting that LTD is indeed mediated by the activation of group II mGluRs. Second, DCG IV-induced LTD was blocked by the NMDA receptor antagonist AP-5, whereas DCG IV did not potentiate NMDA receptor-mediated synaptic responses. Interruption of single test stimuli during DCG IV application blocked DCG IV-induced LTD. These results suggest that small NMDA receptor-mediated responses evoked by single synaptic stimuli contribute to DCG IV-induced LTD. Third, DCG IV-induced LTD was blocked or reduced by the following drugs: phospholipase C inhibitor U-73122 (bath-applied or postsynaptically injected), postsynaptically injected IP3 receptor blocker heparin, phospholipase D-linked mGluR blocker PCCG-13, PKC inhibitor RO318220, postsynaptically injected PKC inhibitor PKC(19-36), and PKA inhibitor KT-5720. Fourth, fluorescent Ca2+ analysis techniques revealed that DCG IV increases Ca2+ concentration in prefrontal layer V pyramidal neurons. These Ca2+ rises and the LTD were both blocked by postsynaptic heparin in the same cells. Taken together, these results suggest that postsynaptic group II mGluRs, linked to phospholipase C and probably also phospholipase D, induce LTD through postsynaptic PKC activation and IP3 receptor-mediated postsynaptic increases of Ca2+ concentration. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://citeseerx.ist.psu.edu/viewdoc/download;jsessionid=3CF787FBD26FBFFACD3F373F1871D558?doi=10.1.1.323.3610&rep=rep1&type=pdf |
| Alternate Webpage(s) | http://www.jneurosci.org/content/22/9/3434.full.pdf |
| PubMed reference number | 11978820v1 |
| Volume Number | 22 |
| Issue Number | 9 |
| Journal | The Journal of neuroscience : the official journal of the Society for Neuroscience |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adrenal Cortex Diseases Calcium ion Carbamoyl-Phosphate Synthase I Deficiency Disease Depressive disorder Esters Glutamate Receptor Glutamic Acid Inositol 1,4,5-Trisphosphate KT 5720 Long-Term Synaptic Depression N-Methyl-D-Aspartate Receptors N-Methylaspartate Neurons PCCG-13 PKA inhibitor Phospholipase C Phospholipase D Prefrontal Cortex Pyramidal Cells Receptors, Metabotropic Glutamate Stage IV Ovarian Carcinoma Synapses U 73122 heparin inositol-1,4,5-triphosphate receptor negative regulation of phospholipase C-activating G-protein coupled receptor signaling pathway negative regulation of type IV pilus biogenesis stage IV cervical cancer |
| Content Type | Text |
| Resource Type | Article |
