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Modulation of NMDA receptor current in layer V pyramidal neurons of the rat prefrontal cortex by P2Y receptor activation.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wirkner, Kerstin Guenther, Albrecht Weber, Marco Guzman, Segundo Jose Krause, Thomas Fuchs, Jochen Köles, László Nörenberg, Wolfgang Illés, Péter |
| Copyright Year | 2007 |
| Abstract | Current responses to N-methyl-D-aspartate (NMDA) in layer V pyramidal neurons of the rat prefrontal cortex were potentiated by the P2 receptor agonists adenosine 5'-triphosphate (ATP) and uridine 5'-triphosphate (UTP). The failure of these nucleotides to induce inward current on fast local superfusion suggested the activation of P2Y rather than P2X receptors. The potentiation by ATP persisted in a Ca(2+)-free superfusion medium but was abolished by 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester, cyclopiazonic acid, 7-nitroindazole, fluoroacetic acid, bafilomycin, and tetanus toxin, indicating that an astrocytic signaling molecule may participate. Because the metabotropic glutamate receptor (mGluR) agonists (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD) (group I/II) and (RS)-3,5-dihydroxyphenylglycine (group I) both imitated the effect of ATP and the group I mGluR antagonist 1-aminoindan-1,5-dicarboxylic acid or a combination of selective mGluR(1) (7-(hydroxyimino)-cyclopropa[b]chromen-1a-carboxylate) and mGluR(5) (2-methyl-6-(phenylethynyl)pyridine) antagonists abolished the facilitation by ATP, it was concluded that the signaling molecule may be glutamate. Pharmacological tools known to interfere with the transduction cascade of type I mGluRs (guanosine 5'-O-(3-thiodiphosphate), U-73122, xestospongin C, 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, calmodulin kinase II [CAMKII] inhibitor peptide) depressed the actions of both ATP and ACPD. Characterization of the P2Y receptor by agonists (ATP and UTP), antagonists (suramin and pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid), and knockout mice (P2Y(2)(-/-)) suggested that the nucleotides act at the P2Y(4) subtype. In conclusion, we propose that exogenous and probably also endogenous ATP release vesicular glutamate from astrocytes by P2Y(4) receptor activation. This glutamate then stimulates type I mGluRs of layer V pyramidal neurons and via the G(q)/phospholipase C/inositol 1,4,5-trisphosphate/Ca(2+)/CAMKII transduction pathway facilitates NMDA receptor currents. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://cercor.oxfordjournals.org/content/early/2006/04/28/cercor.bhk012.full.pdf |
| Alternate Webpage(s) | http://cercor.oxfordjournals.org/content/17/3/621.full.pdf |
| Alternate Webpage(s) | https://watermark.silverchair.com/bhk012.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAaQwggGgBgkqhkiG9w0BBwagggGRMIIBjQIBADCCAYYGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM7lJ8hJHLcTJ3aCMJAgEQgIIBV6ylKdCY66E0qEacWNE_llQ13z76DUvtzul8x1tzAUW6upFBgSNnI60YYzpkl5NMVqFNjr1Qy7_GTqcx_FQXRkLLcvZXpBXwL6fBnbjCE820oKqJuwbZV2S4gdANt46Qa7Z4PVuKKjfOM5Mm6doqH3DUe6SIibU42B6o52Hma5dotnnHNI1W_5PftMF9ZZNf4otUrUju0kDUy_-OB2eTuBWTCBsJkxlQ_FkNA2OoW_f3vJs3rnuAY5ztgTnzHchk9ks7f1--HyXVzZdLV7YcXwWWN2AYkybwRkUU4XN8wtUtkSbjE5VtL3XLxq8ZckSYxw730U9H-6d5ZTUOhEkTHajVJKzo5Tqj13Tnv8O86vxNuexLRAqXxxhntHxgNBoki72mJccksgdVWf-WQ31l3A1rHbzJ2YwZAlebyAowTNi6V_84Mvgx0A_VN1jgCl56am489cB4-5U |
| PubMed reference number | 16648456v1 |
| Volume Number | 17 |
| Issue Number | 3 |
| Journal | Cerebral cortex |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 1-amino-1,3-dicarboxycyclopentane 1-aminoindan-1,5-dicarboxylic acid 6-methyl-2-(phenylethynyl)pyridine 7-nitroindazole Adenosine Triphosphate Aspartic Acid Calcium-Calmodulin-Dependent Protein Kinases Cascade Device Component Depressive disorder Dopaminergic Neurons Esters G-protein coupled purinergic nucleotide receptor activity Glutamate Receptor Glutamic Acid Guanosine Inositol 1,4,5-Trisphosphate N-Methyl-D-Aspartate Receptors N-Methylaspartate Nucleotides Pharmacology Prefrontal Cortex Pyramidal Cells Pyridoxal Receptor Activation Process Receptors, Metabotropic Glutamate Suramin Tetany Thioctic Acid U 73122 Uridine Triphosphate carboxylate chemosensitization/potentiation cyclopiazonic acid facilitation fluoroacetic acid tetanus toxin transduction |
| Content Type | Text |
| Resource Type | Article |
