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Calcitonin gene-related peptide-like immunoreactivity in the central nervous system and peripheral organs of rats
| Content Provider | Semantic Scholar |
|---|---|
| Author | Okimura, Yasuhiko Chihara, Kazuo Abe, Hiromi Kita, Tetsuya Fujita, Takuo |
| Copyright Year | 1987 |
| Abstract | The concentrations of rat calcitonin gene-related peptide-like immunoreactivity (rCGRP-LI) in various organs of male rats as well as the molecular heterogeneity of rCGRP-LI in tissue extracts was examined using a specific radioimmunoassay (RIA) for rCGRP and gel-filtration chromatography. rCGRP-LI was high in extracts of the spinal cord (202 +/- 22.6 pg/mg wet wt. of tissue; mean +/- S.E.M.) and of the thyroid (229 +/- 62.3 pg/mg). rCGRP-LI was detectable in the brainstem, hypothalamus, stomach, duedenum, pancreas and kidney. The elution pattern of the extracts on a Sephadex G-50 column showed 3 peaks of rCGRP-LI irrespective of organs and tissues. The first peak corresponded to authentic rCGRP-(1-37). The second and third rCGRP-LI peaks probably consisted of C-terminal fragments of rCGRP, because they had a lower molecular weight than rCGRP-(1-37) and because our antiserum cross-reacts with a synthetic C-terminal fragment. The ratio of 3 rCGRP-LI molecules, however, differed between neural tissue extracts and others. The main component of rCGRP-LI in neural tissue was authentic rCGRP-(1-37), while the smaller fragments of rCGRP were chiefly contained in other tissues like the stomach, pancreas and thyroid. The relative ratio of rCGRP-LI molecules with different size in respective tissue extracts was not changed after leaving the dissected tissues for 2 h at room temperature. These findings indicate that rCGRP-LI is abundantly present in the thyroid as well as the spinal cord and it is detected in lower amounts in the alimentary tract and central nervous system. rCGRP-LI in the extracts consists of 3 different components, the proportions of which vary from one tissue to another, probably reflecting tissue-specific differences in the processing of CGRP. |
| Starting Page | 327 |
| Ending Page | 337 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0167-0115(87)90056-5 |
| PubMed reference number | 3496628 |
| Journal | Medline |
| Volume Number | 17 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0167011587900565 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0167011587900565?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0167-0115%2887%2990056-5 |
| Journal | Regulatory Peptides |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
