NDLI: Reactive phosphate ester of the carcinogen 2-(N-hydroxy)acetamido-fluorene.

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Reactive phosphate ester of the carcinogen 2-(N-hydroxy)acetamido-fluorene.

Content Provider	Semantic Scholar
Author	Lotlikar, Prabhakar D. Wasserman, Michelle C.
Copyright Year	1970
Abstract	1. Reaction of 2-(N-acetoxy)-acetamidofluorene with orthophosphate buffer at pH7 yielded a large quantity of water-soluble fluorene derivatives, which showed absorption peaks at 303, 290 and 280nm. Tris buffer under similar conditions gave negligible reaction. 2. Hydrolysis of polar material with acid or alkaline phosphatases liberated equimolar amounts of inorganic phosphate and an ether-extractable fluorene derivative. On the basis of its u.v. spectrum, R(F) values after paper chromatography, solubility in alkali and colour with spray reagents, the derivative was characterized tentatively as 2-acetamido-5-hydroxyfluorene. 3. Polar material also contained a reactive fluorene derivative which gave characteristic reaction products with methionine and guanosine. The reactive derivative was characterized as a phosphate ester of 2-(N-hydroxy)-acetamidofluorene. 4. It is suggested that such reactive phosphate esters may also be some of the ultimate carcinogenic metabolites of carcinogenic aromatic hydroxamic acids.
Starting Page	313
Ending Page	313
Page Count	1
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.biochemj.org/content/ppbiochemj/120/3/661.full.pdf
PubMed reference number	5499979v1
Volume Number	120
Issue Number	3
Journal	The Biochemical journal
Language	English
Access Restriction	Open
Subject Keyword	AROMATICS Alkalies Alkaline Phosphatase Buffers Carbamoyl-Phosphate Synthase I Deficiency Disease Carcinogens Contain (action) Esters Ethers Fatty Acids, Omega-6 Guanosine Hydroxamic Acids Metabolite Methionine Orthophosphate Paper Chromatography (procedure) Phosphate measurement Phosphoric Monoester Hydrolases Reagents Tris(2,3-dibromopropyl) Phosphate Tromethamine fluorene inorganic phosphate phosphate ester
Content Type	Text
Resource Type	Article

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