Fluorescence and delayed light emission in Tris-washed chloroplasts.

Content Provider	Semantic Scholar
Author	Mohanty, P. Braun, Bettina Govindjee
Copyright Year	1972
Abstract	Yamashita and Butler [I] have shown that extraction of chloroplasts with a high concentration of Tris (0.8 M, pH 8.0) eliminates flow of electrons from water to nicotinamide adenine dinucleotide phosphate (NADP+)* or other Hill oxidants. They suggest that washing with Tris causes a block on the water side of the electron transport chain. Addition of electron donors such as reduced phenylene diamine (PDA) restores the electron flow to NADP’. This donation of electrons by PDA is sensitive to the herbicide diuron (DCMU), a well known inhibitor of photosystem II (PS II) reactions. Measurements of chlorophyll a (Chl) fluorescence in Tris-washed chloroplasts (without any added donor) show a very low level of fluorescence, but the addition of DCMU increases the yield of fluorescence [I]. One could suggest that the low yield of Chl fluorescence in Tris-washed chloroplasts is due to “trickling” of electron flow from some endogenous donor (cf. [ 11). However, if this were so, we should expect the fluorescence yield to slowly rise and reach the maximum yield at longer times. But, this never happens. No satisfactory explanation was available for this peculiar behavior of fluorescence
Starting Page	273
Ending Page	276
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.life.illinois.edu/govindjee/Electronic%20Publications/1972/1972_mohanty_et_al_FEBS%20Lett.%20(1972).pdf
PubMed reference number	11946436v1
Volume Number	20
Issue Number	3
Journal	FEBS letters
Language	English
Access Restriction	Open
Subject Keyword	Adenine Carbamoyl-Phosphate Synthase I Deficiency Disease Chloroplasts Diamine oxidase:ACnc:Pt:Ser/Plas:Qn Diamines Dinucleoside Phosphates Diuron Electrons Fluorescence Herbicides Hodgkin Disease NADP Niacin Oxidants Photosystem II Tris(2,3-dibromopropyl) Phosphate Tromethamine Wash (cleansing action) chlorophyll a electron transport chain light emission
Content Type	Text
Resource Type	Article