Loading...
Please wait, while we are loading the content...
Similar Documents
Activation of the alternative complement pathway by fungal melanins.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rosas, Angel L. Macgill, Randall S. Nosanchuk, Joshua D. Kozel, Thomas R. Casadevall, Arturo |
| Copyright Year | 2002 |
| Abstract | Melanins are complex biological pigments formed by the oxidative polymerization of phenolic and/or indolic compounds. These pigments have been implicated in the pathogenesis of some microbial infections, malignancies, degenerative disorders, and autoimmune diseases. Recent studies have demonstrated that melanins have antigenic and anti-inflammatory properties. These findings led us to further explore the interaction of melanins with the immune system. Melanin particles ("ghosts") were isolated from in vitro-melanized Cryptococcus neoformans cells and Aspergillus niger conidia and then incubated in normal human serum containing (125)I-labeled complement C3. The results demonstrated deposition of C3 fragments onto the melanin ghosts as early as 1 min after incubation, with maximum deposition occurring after 12 min for C. neoformans-derived melanin ghosts and after 25 min for A. niger-derived melanin ghosts. The blocking of classical pathway activation did not affect the kinetics or total deposition of C3 onto the melanin ghosts, indicating that melanins activate complement through the alternative pathway. Immunofluorescence analysis of lungs from BALB/c mice injected intratracheally with C. neoformans-derived melanin ghosts demonstrated deposition of C3 fragments onto the ghosts. Small granulomas were also observed surrounding the ghosts. However, melanization of the C. neoformans cell wall did not alter the kinetics or total deposition of C3 fragments onto the fungal cells. The finding that melanin surfaces can activate the complement system suggests a potential mechanism for the pathogenesis of some degenerative and/or autoimmune processes that involve melanized cells as well as another potential role for melanin in the virulence of melanin-producing microorganisms. |
| Starting Page | 1 |
| Ending Page | 1 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://cvi.asm.org/content/9/1/144.full.pdf |
| PubMed reference number | 11777844v1 |
| Volume Number | 9 |
| Issue Number | 1 |
| Journal | Clinical and diagnostic laboratory immunology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Abnormal degeneration Activation action Autoimmune Diseases Cell Wall Complement Pathway, Alternative Complement System Proteins Cryptococcus neoformans Ab:ACnc:Pt:Ser:Qn Cryptococcus neoformans:PrThr:Pt:XXX:Ord:Organism specific culture Granuloma Infection Lung Melanins Microorganism Pigments, Biological Tracer Virulence complement pathway degenerative disorder incubated nervous system disorder |
| Content Type | Text |
| Resource Type | Article |
