NDLI: An efficiently regulated promoter system for Cryptococcus neoformans utilizing the CTR4 promoter.

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An efficiently regulated promoter system for Cryptococcus neoformans utilizing the CTR4 promoter.

Content Provider	Semantic Scholar
Author	Ory, Jeramia Griffith, Cara L. Doering, Tamara L.
Copyright Year	2004
Abstract	Cryptococcus neoformans is an opportunistic fungal pathogen responsible for serious meningitis. Although many useful molecular tools have been developed for its study, there are currently few inducible promoters available for general use. To address this need, we have constructed expression plasmids incorporating upstream elements of the C. neoformans copper transporter gene CTR4, and tested them in C. neoformans serotypes A and D. In response to copper deprivation, these plasmids mediate high-level expression of a reporter protein. This expression can be completely repressed using physiologically low concentrations of copper. Notably, this new family of copper-sensing promoters demonstrates excellent expression in serotype A, contrasting with other available promoters. These plasmids therefore offer efficient and regulated expression for both serotypes A and D, and should be valuable tools for the C. neoformans research community.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.crypto.wustl.edu/Reprints/Ory%20et%20al%20Yeast%202004.pdf
PubMed reference number	15334556v1
Volume Number	21
Issue Number	11
Journal	Yeast
Language	English
Access Restriction	Open
Subject Keyword	Cryptococcus neoformans Ab:ACnc:Pt:Ser:Qn Cryptococcus neoformans:PrThr:Pt:XXX:Ord:Organism specific culture Meningitis Pathogenic organism Plasmids Serotype promoter response to copper ion
Content Type	Text
Resource Type	Article

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