NDLI: The addition of endothelial cell growth factor and heparin to human umbilical vein endothelial cell cultures decreases plasminogen activator inhibitor-1 expression.

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The addition of endothelial cell growth factor and heparin to human umbilical vein endothelial cell cultures decreases plasminogen activator inhibitor-1 expression.

Content Provider	Semantic Scholar
Author	Konkle, Barbara A. Ginsburg, David
Copyright Year	1988
Abstract	Plasminogen activator inhibitor-1 (PAI-1) is a specific and rapid inhibitor of tissue plasminogen activator (tPA) and urokinase. Clinical studies suggest that PAI-1 may play a crucial role in the regulation of fibrinolysis. A number of factors modulate PAI-1 activity in endothelial cell culture, and the isolation of PAI-1 cDNA now allows study of PAI-1 regulation at the mRNA level. We examined the effect of endothelial cell growth factor (ECGF) and heparin on PAI-1 expression in human umbilical vein endothelial cell (HUVEC) culture. The addition of ECGF and heparin to HUVEC cultures results in a 3-10-fold decrease in the PAI-1 activity secreted into the conditioned media. This effect is mediated at the mRNA level. A decrease in PAI-1 is also seen with higher concentrations of ECGF alone, but is greatly enhanced by the addition of heparin. No significant change in tPA antigen or mRNA levels was observed.
File Format	PDF HTM / HTML
DOI	10.1172/JCI113635
Alternate Webpage(s)	https://dm5migu4zj3pb.cloudfront.net/manuscripts/113000/113635/cache/113635.1-20150304082311-covered-253bed37ca4c1ab43d105aefdf7b5536.pdf
Alternate Webpage(s)	https://www.jci.org/articles/view/113635/pdf/render
Alternate Webpage(s)	http://dm5migu4zj3pb.cloudfront.net/manuscripts/113000/113635/JCI88113635.pdf
PubMed reference number	3136192
Alternate Webpage(s)	https://doi.org/10.1172/JCI113635
Journal	Medline
Volume Number	82
Issue Number	2
Journal	The Journal of clinical investigation
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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