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Influence of Mechanical Force on Bone Matrix Proteins in Ovariectomised Mice and Osteoblast-like MC3T3-E1 Cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Meng Ishikawa, Shintaro Inagawa, Tomoko Ikemoto, Hideshi Guo, Shiyu Sunagawa, Masataka Hisamitsu, Tadashi |
| Copyright Year | 2017 |
| Abstract | AIM To investigate the effect of mechanical stress on periostin and semaphorin-3A expression in a murine model of postmenopausal osteoporosis and in osteoblast-like MC3T3-E1 cells. MATERIALS AND METHODS Female mice were divided into three groups and treated with a sham operation, ovariectomy (OVX) or OVX plus treadmill training (OVX+Run). After 10 weeks, tibias were used for histological analysis. MC3T3-E1 cells were burdened by mechanical stress using a centrifuge or were treated with periostin, and the production of biologically-active semaphorin-3A was examined in vitro. RESULTS In OVX+Run group tibias, the number of tartrate-resistant acid phosphatase-positive osteoclasts was lower than in the OVX group, and the expression of periostin and semaphorin-3A was higher. In MC3T3-E1 cells, centrifugal stress significantly increased periostin and semaphorin-3A mRNA expression. Treatment with periostin increased the semaphorin-3A level. CONCLUSION We speculate that mechanical load may increase periostin production in osteoblasts, and periostin may inhibit osteoclast differentiation by its effects on semaphorin-3A. Our results support the concept of a positive correlation between exercise and inhibition of osteoclasts in post-menopausal osteoporosis. |
| Starting Page | 87 |
| Ending Page | 95 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://iv.iiarjournals.org/content/31/1/87.full.pdf |
| PubMed reference number | 28064225v1 |
| Volume Number | 31 |
| Issue Number | 1 |
| Journal | In vivo |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | ACP5 gene Acid Phosphatase Bone Marrow Diseases Bone structure of tibia Menopause Osteoblasts Osteoclasts Osteoporosis Osteoporosis, Postmenopausal Ovariectomy POSTN gene Semaphorin-3A Stress, Mechanical Tartrates Treadmill, device osteoclast differentiation |
| Content Type | Text |
| Resource Type | Article |
