NDLI: Mangiferin positively regulates osteoblast differentiation and suppresses osteoclast differentiation

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Mangiferin positively regulates osteoblast differentiation and suppresses osteoclast differentiation

Content Provider	Semantic Scholar
Author	Sekiguchi, Yuusuke Mano, Hiroshi Nakatani, Sachie Shimizu, Jun Kataoka, Aya Ogura, Kh. Kimira, Yoshifumi Ebata, Midori Wada, Masahiro
Copyright Year	2017
Abstract	Mangiferin is a polyphenolic compound present in Salacia reticulata. It has been reported to reduce bone destruction and inhibit osteoclastic differentiation. This study aimed to determine whether mangiferin directly affects osteoblast and osteoclast proliferation and differentiation, and gene expression in MC3T3‑E1 osteoblastic cells and osteoclast‑like cells derived from primary mouse bone marrow macrophage cells. Mangiferin induced significantly greater WST‑1 activity, indicating increased cell proliferation. Mangiferin induced significantly increased alkaline phosphatase staining, indicating greater cell differentiation. Reverse transcription‑polymerase chain reaction (RT‑PCR) demonstrated that mangiferin significantly increased the mRNA level of runt‑related transcription factor 2 (RunX2), but did not affect RunX1 mRNA expression. Mangiferin significantly reduced the formation of tartrate‑resistant acid phosphatase‑positive multinuclear cells. RT‑PCR demonstrated that mangiferin significantly increased the mRNA level of estrogen receptor β (ERβ), but did not affect the expression of other osteoclast‑associated genes. Mangiferin may inhibit osteoclastic bone resorption by suppressing differentiation of osteoclasts and promoting expression of ERβ mRNA in mouse bone marrow macrophage cells. It also has potential to promote osteoblastic bone formation by promoting cell proliferation and inducing cell differentiation in preosteoblast MC3T3‑E1 cells via RunX2. Mangiferin may therefore be useful in improving bone disease outcomes.
Starting Page	1328
Ending Page	1332
Page Count	5
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.spandidos-publications.com/10.3892/mmr.2017.6752/download
PubMed reference number	28627701v1
Alternate Webpage(s)	https://doi.org/10.3892/mmr.2017.6752
DOI	10.3892/mmr.2017.6752
Journal	Molecular medicine reports
Volume Number	16
Language	English
Access Restriction	Open
Subject Keyword	Alkaline Phosphatase Bone Diseases Bone Marrow Bone Resorption Cell Differentiation process Cell Proliferation Estrogens Gene Expression Hepatocyte Nuclear Factor 1-beta Immunoglobulin kappa-Chains Osteoblasts Osteoclasts Osteogenesis Promotion (action) Salacia TRANSCRIPTION FACTOR Thioctic Acid estrogen receptor alpha, human mangiferin osteoblast differentiation osteoclast differentiation osteoclast proliferation pars reticulata of substantia nigra
Content Type	Text
Resource Type	Article

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