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The Threonine-Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K12. Distribution and Accessibility to Antibodies of Some Epitopes of the Bifunctional Enzyme
| Content Provider | Scilit |
|---|---|
| Author | Costrejean, Jean-Marc Guiso, Nicole Cowie, Dean B. Cohen, Georges N. Truffa-Bachi, Paolo |
| Copyright Year | 1975 |
| Description | Journal: Journal of Biological Inorganic Chemistry In the presence of l-threonine, the allosteric effector, most of the antigenic determinants situated in the aspartokinase region of the wild-type enzyme become unavailable to the antibodies raised against a fragment of the enzyme containing this region and devoid of homoserine dehydrogenase activity. The cross-reactivities of the antibodies raised against this fragment (extracted from a nonsense mutant) and a fragment endowed with homoserine dehydrogenase activity but devoid of aspartokinase activity (obtained by limited proteolysis) with the corresponding antigens were studied. The conclusion is drawn that the two fragments, which share an overlapping sequence of molecular weight about 17,000, share at least two antigenic determinants. |
| Ending Page | 435 |
| Starting Page | 431 |
| ISSN | 2573508X |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1975.tb09820.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 2 |
| Volume Number | 50 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1975-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Homoserine Dehydrogenase Aspartokinase Activity Dehydrogenase Activity Antigenic Determinants Antibodies Raised |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
