NDLI: The Threonine-Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K12. Specific Inactivation of the Homoserine Dehydrogenase Activity by the Affinity Label, 2-Amino-4-oxo-5-chloropentanoic Acid

Please wait, while we are loading the content...

The Threonine-Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K12. Specific Inactivation of the Homoserine Dehydrogenase Activity by the Affinity Label, 2-Amino-4-oxo-5-chloropentanoic Acid

Content Provider	Scilit
Author	Hirth, Christian G. Veron, Michel Villar-Palasi, Carlos Hurion, Nicole Cohen, Georges N.
Copyright Year	1975
Description	Journal: Journal of Biological Inorganic Chemistry 2-Amino-4-oxo-5-chloropentanoic acid inactivates specifically the homoserine dehydrogenase activity of the bifunctional enzyme, aspartokinase I--homoserine dehydrogenase I. The aspartokinase activity remains essentially untouched and retains its threonine sensitivity. The inactivation of the dehydrogenase requires the covalent binding of one equivalent of the analogue per subunit. Alkylation does not affect the tetrameric state of the protein. The alkylating agent, a substrate analogue, meets the qualitative and quantitative requirements of an affinity label.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1975.tb09819.x/pdf
Ending Page	430
Page Count	6
Starting Page	425
ISSN	09498257
e-ISSN	14321327
DOI	10.1111/j.1432-1033.1975.tb09819.x
Journal	Journal of Biological Inorganic Chemistry
Issue Number	2
Volume Number	50
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1975-01-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Homoserine Dehydrogenase
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in