NDLI: The Threonine-Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K-12. Incubation of the Enzyme in Alkaline Conditions: Dissociation and Disulfide-Bridge Formation

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The Threonine-Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K-12. Incubation of the Enzyme in Alkaline Conditions: Dissociation and Disulfide-Bridge Formation

Content Provider	Scilit
Author	Jacques, Yannick Truffa‐Bachi, Paolo
Copyright Year	1976
Description	Journal: Journal of Biological Inorganic Chemistry Aspartokinase I - homoserine dehydrogenase I from Escherichia coli K-12, a homotetrameric enzyme, dissociates into dimers upon alkaline treatment. Both aspartokinase and homoserine dehydrogenase inactivation, as well as desensitazion towards L-threonine, occur in a multi-step process. Dithiothreitol stabilizes a dimeric form retaining full activity and sensitivity; L-homoserine stabilizing another dimeric form devoid of aspartokinase activity and retaining a substantial dehydrogenase activity insensitive toward L-threonine. A model is proposed showing that dissociation into dimers occurs in a first step, the resulting dimer losing both aspartokinase and homoserine dehydrogenase sensitivity in two subsequent steps involving the formation of intrachain disulfide bonds.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1976.tb10182.x/pdf
Ending Page	490
Page Count	6
Starting Page	485
ISSN	09498257
e-ISSN	14321327
DOI	10.1111/j.1432-1033.1976.tb10182.x
Journal	Journal of Biological Inorganic Chemistry
Issue Number	3
Volume Number	62
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1976-03-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Homoserine Dehydrogenase Escherichia Coli Coli K Disulfide Bridge Bridge Formation Threonine Sensitive Sensitive Homoserine Aspartokinase Activities
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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The Threonine-Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K 12. A Study of the Allosteric Equilibrium

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