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Discrepancies between the phenotypic and genotypic characterization of Lactococcus lactis cheese isolates
| Content Provider | Scilit |
|---|---|
| Author | Plaza, M. De La Rodríguez, A. Palencia, P. Fernández De Martínez‐Cuesta, M. C. Peláez, C. Requena, Teresa |
| Copyright Year | 2006 |
| Description | Journal: Letters in applied microbiology 8 páginas, 4 figuras,2 tablasAims: The use of randomly amplified polymorphic DNA (RAPD)-PCR fingerprinting and plasmid profiles to determine at the strain level, the similarity of Lactococcus lactis isolates obtained during sampling of traditional cheeses and to verify its correspondence to the selected phenotypic characteristics. Methods and Results: A total of 45 L. lactis isolates were genotypically analysed by RAPD-PCR fingerprinting and plasmid patterns. Phenotypic traits used to compare strains were proteolytic, acidifying, aminotransferase (aromatic and branched chain aminotransferase) and α-ketoisovalerate decarboxylase (Kivd) activities. The results show that 23 isolates could be grouped in clusters that exhibited 100% identity in both their RAPD and plasmid patterns, indicating the probable isolation of dominant strains during the cheese sampling process. However, there were phenotypic differences between isolates within the same cluster that included the loss of relevant technological properties such as proteinase activity and acidifying capacity or high variation in their amino acid converting enzyme activities. Likewise, the analysis of a specific attribute, Kivd activity, indicated that 7 of 15 isolates showed no detectable activity despite the presence of the encoding (kivd) gene. Conclusion: Phenotypic differences found between genotypically similar strains of L. lactis strains could be linked to differences in enzymatic expression. Significance and Impact of the Study: Phenotypic analysis of L. lactis isolates should be considered when selecting strains with new cheese flavour forming capabilities. © 2006 The Authors.This work was financed by the Spanish National Research project AGL 2002-03277.Peer reviewe |
| Related Links | https://core.ac.uk/reader/36181576 http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2006.02012.x/pdf |
| Ending Page | 644 |
| Page Count | 8 |
| Starting Page | 637 |
| e-ISSN | 1472765X |
| DOI | 10.1111/j.1472-765x.2006.02012.x |
| Journal | Letters in applied microbiology |
| Issue Number | 6 |
| Volume Number | 43 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2006-12-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Letters in applied microbiology Food Science and Technology Amino Acid Catabolism Lactococcus Lactis Wild Strains |
| Content Type | Text |
| Resource Type | Article |
