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Persistence ofEscherichia coliO157:H7 on the rhizosphere and phyllosphere of lettuce
| Content Provider | Scilit |
|---|---|
| Author | Ibekwe, A. Mark Grieve, C. M. Papiernik, S. K. Yang, C. -H. |
| Copyright Year | 2009 |
| Description | Journal: Letters in applied microbiology Aims: The major objective of this study was to determine the effects of low levels of Escherichia coli O157:H7 contamination on plant by monitoring the survival of the pathogen on the rhizosphere and leaf surfaces of lettuce during the growth process. Methods and Results: Real-time PCR and plate counts were used to quantify the survival of E. coli O157:H7 in the rhizosphere and leaf surfaces after planting. Real-time PCR assays were designed to amplify the stx1, stx2 and the eae genes of E. coli O157:H7. The detection limit for E. coli O157:H7 quantification by real-time PCR was 2·4 × $10^{3}$ CFU $g^{−1}$ of starting DNA in rhizosphere and phyllosphere samples and about $10^{2}$ CFU $g^{−1}$ by plate count. The time for pathogens to reach detection limits on the leaf surface by plate counts was 7 days after planting in comparison with 21 days in the rhizosphere. However, real-time PCR continued to detect stx1, stx2 and the eae genes throughout the experimental period. Conclusion: Escherichia coli O157:H7 survived throughout the growth period as was determined by real-time PCR and by subsequent enrichment and immunomagnetic separation of edible part of plants. Significance and impact of the Study: The potential presence of human pathogens in vegetables grown in soils contaminated with E. coli O157:H7 is a serious problem to our national food supply as the pathogen may survive on the leaf surface as they come in contact with contaminated soil during germination. |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1472-765X.2009.02745.x |
| Ending Page | 790 |
| Page Count | 7 |
| Starting Page | 784 |
| e-ISSN | 1472765X |
| DOI | 10.1111/j.1472-765x.2009.02745.x |
| Journal | Letters in applied microbiology |
| Issue Number | 6 |
| Volume Number | 49 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2009-11-10 |
| Access Restriction | Open |
| Subject Keyword | Journal: Letters in applied microbiology Food Science and Technology Veterinary Sciences Escherichia Coli O157:h7 |
| Content Type | Text |
| Resource Type | Article |
