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Biocontrol ofEscherichia coliO157
| Content Provider | Scilit |
|---|---|
| Author | Boyacioglu, Olcay Sharma, Manan Sulakvelidze, Alexander Goktepe, Ipek |
| Copyright Year | 2013 |
| Description | Journal: Bacteriophage The effect of a bacteriophage cocktail (EcoShield™) that is specific against Escherichia coli O157:H7 was evaluated against a nalidixic acid-resistant enterohemorrhagic E. coli O157:H7 RM4407 (EHEC) strain on leafy greens stored under either (1) ambient air or (2) modified atmosphere (MA; 5% $O_{2}$/35% $CO_{2}$/60% $N_{2}$). Pieces (~2 × 2 $cm^{2}$) of leafy greens (lettuce and spinach) inoculated with 4.5 log $CFU/cm^{2}$ EHEC were sprayed with EcoShield™ (6.5 log $PFU/cm^{2}$). Samples were stored at 4 or 10°C for up to 15 d. On spinach, the level of EHEC declined by 2.38 and 2.49 log $CFU/cm^{2}$ at 4 and 10°C, respectively, 30 min after phage application (p ≤ 0.05). EcoShield™ was also effective in reducing EHEC on the surface of green leaf lettuce stored at 4°C by 2.49 and 3.28 log units in 30 min and 2 h, respectively (p ≤ 0.05). At 4°C under atmospheric air, the phage cocktail significantly (p ≤ 0.05) lowered the EHEC counts in one day by 1.19, 3.21 and 3.25 log $CFU/cm^{2}$ on spinach, green leaf and romaine lettuce, respectively compared with control (no bacteriophage) treatments. When stored under MA at 4°C, phages reduced (p ≤ 0.05) EHEC populations by 2.18, 3.50 and 3.13 log $CFU/cm^{2}$, on spinach, green leaf and romaine lettuce. At 10°C, EHEC reductions under atmospheric air storage were 1.99, 3.90 and 3.99 log $CFU/cm^{2}$ (p ≤ 0.05), while population reductions under MA were 3.08, 3.89 and 4.34 logs on spinach, green leaf and romaine lettuce, respectively, compared with controls (p ≤ 0.05). The results of this study showed that bacteriophages were effective in reducing the levels of E. coli O157:H7 on fresh leafy produce, and that the reduction was further improved when produce was stored under the MA conditions. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.4161/bact.24620?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694058/pdf |
| ISSN | 21597073 |
| e-ISSN | 21597081 |
| DOI | 10.4161/bact.24620 |
| Journal | Bacteriophage |
| Issue Number | 1 |
| Volume Number | 3 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2013-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Bacteriophage Food Science and Technology E. Coli O157:h7 Bacteriophage Modified Atmosphere Packaging |
| Content Type | Text |
