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Comprehensive evaluation of the bactericidal activities of free bile acids in the large intestine of humans and rodents
| Content Provider | Scilit |
|---|---|
| Author | Watanabe, Masamichi Fukiya, Satoru Yokota, Atsushi |
| Copyright Year | 2017 |
| Description | Journal: Journal of Lipid Research In addition to functioning as detergents that aid digestion of dietary lipids in the intestine, some bile acids have been shown to exhibit antimicrobial activity. However, detailed information on the bactericidal activities of the diverse molecular species of bile acid in humans and rodents is largely unknown. Here, we investigated the toxicity of 14 typical human and rodent free bile acids (FBAs) by monitoring intracellular pH, membrane integrity, and viability of a human intestinal bacterium, Bifidobacterium breve Japan Collection of Microorganisms (JCM) 1192T, upon exposure to these FBAs. Of all FBAs evaluated, deoxycholic acid (DCA) and chenodeoxycholic acid displayed the highest toxicities. Nine FBAs common to humans and rodents demonstrated that α-hydroxy-type bile acids are more toxic than their oxo-derivatives and β-hydroxy-type epimers. In five rodent-specific FBAs, β-muricholic acid and hyodeoxycholic acid showed comparable toxicities at a level close to DCA. Similar trends were observed for the membrane-damaging effects and bactericidal activities to Blautia coccoides JCM 1395T and Bacteroides thetaiotaomicron DSM 2079T, commonly represented in the human and rodent gut microbiota. These findings will help us to determine the fundamental properties of FBAs and better understand the role of FBAs in the regulation of gut microbiota composition. |
| Related Links | http://www.jlr.org/content/58/6/1143.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454512/pdf |
| Ending Page | 1152 |
| Page Count | 10 |
| Starting Page | 1143 |
| ISSN | 00222275 |
| e-ISSN | 15397262 |
| DOI | 10.1194/jlr.m075143 |
| Journal | Journal of Lipid Research |
| Issue Number | 6 |
| Volume Number | 58 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2017-06-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Lipid Research Bile Acids and Salts Bile Acids and Salts/metabolism Bile Acids and Salts/physical Chemistry Deoxycholic Acid Gut Microbiota Hyodeoxycholic Acid Β-muricholic Acid |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Endocrinology |
