NDLI: NuSAP, a novel microtubule-associated protein involved in mitotic spindle organization

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NuSAP, a novel microtubule-associated protein involved in mitotic spindle organization

Content Provider	Scilit
Author	Raemaekers, Tim Ribbeck, Katharina Beaudouin, Joël Annaert, Wim Camp, Mark Van Stockmans, Ingrid Smets, Nico Bouillon, Roger Ellenberg, Jan Carmeliet, Geert
Copyright Year	2003
Description	Here, we report on the identification of nucleolar spindle–associated protein (NuSAP), a novel 55-kD vertebrate protein with selective expression in proliferating cells. Its mRNA and protein levels peak at the transition of G2 to mitosis and abruptly decline after cell division. Microscopic analysis of both fixed and live mammalian cells showed that NuSAP is primarily nucleolar in interphase, and localizes prominently to central spindle microtubules during mitosis. Direct interaction of NuSAP with microtubules was demonstrated in vitro. Overexpression of NuSAP caused profound bundling of cytoplasmic microtubules in interphase cells, and this relied on a COOH-terminal microtubule-binding domain. In contrast, depletion of NuSAP by RNA interference resulted in aberrant mitotic spindles, defective chromosome segregation, and cytokinesis. In addition, many NuSAP-depleted interphase cells had deformed nuclei. Both overexpression and knockdown of NuSAP impaired cell proliferation. These results suggest a crucial role for NuSAP in spindle microtubule organization.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172854/pdf http://jcb.rupress.org/content/162/6/1017.full.pdf
Ending Page	1029
Page Count	13
Starting Page	1017
DOI	10.1083/jcb.200302129
Journal	Journal of Cell Biology
Issue Number	6
Volume Number	162
Language	English
Publisher	Rockefeller University Press
Publisher Date	2003-09-08
Access Restriction	Open
Subject Keyword	Cell Biology Mitotic Spindle Apparatus; Microtubules; Nucleus; Mitosis; Nucleolus Journal: Journal of Cell Biology (Vol- 162, Issue- 6)
Content Type	Text

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