NDLI: NuSAP, a novel microtubule-associated protein involved in mitotic spindle organization

Content Provider	PubMed Central
Author	Raemaekers, Tim Ribbeck, Katharina Beaudouin, Joël Annaert, Wim Camp, Mark Van Stockmans, Ingrid Smets, Nico Bouillon, Roger Ellenberg, Jan Carmeliet, Geert
Abstract	Here, we report on the identification of nucleolar spindle–associated protein (NuSAP), a novel 55-kD vertebrate protein with selective expression in proliferating cells. Its mRNA and protein levels peak at the transition of G2 to mitosis and abruptly decline after cell division. Microscopic analysis of both fixed and live mammalian cells showed that NuSAP is primarily nucleolar in interphase, and localizes prominently to central spindle microtubules during mitosis. Direct interaction of NuSAP with microtubules was demonstrated in vitro. Overexpression of NuSAP caused profound bundling of cytoplasmic microtubules in interphase cells, and this relied on a COOH-terminal microtubule-binding domain. In contrast, depletion of NuSAP by RNA interference resulted in aberrant mitotic spindles, defective chromosome segregation, and cytokinesis. In addition, many NuSAP-depleted interphase cells had deformed nuclei. Both overexpression and knockdown of NuSAP impaired cell proliferation. These results suggest a crucial role for NuSAP in spindle microtubule organization.
Related Links	http://dx.doi.org/10.1083/jcb.200302129
Starting Page	1017
File Format	PDF
ISSN	15408140
e-ISSN	15408140
Journal	The Journal of Cell Biology
Issue Number	6
Volume Number	162
Language	English
Publisher	The Rockefeller University Press
Publisher Date	2003-09-15
Access Restriction	Open
Rights Holder	The Rockefeller University Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine

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