NDLI: Saccharomyces cerevisiae Duo1p and Dam1p, Novel Proteins Involved in Mitotic Spindle Function

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Saccharomyces cerevisiae Duo1p and Dam1p, Novel Proteins Involved in Mitotic Spindle Function

Content Provider	Scilit
Author	Hofmann, Christian Cheeseman, Iain Goode, Bruce L. McDonald, Kent L. Barnes, Georjana Drubin, David G.
Copyright Year	1998
Description	In this paper, we describe the identification and characterization of two novel and essential mitotic spindle proteins, Duo1p and Dam1p. Duo1p was isolated because its overexpression caused defects in mitosis and a mitotic arrest. Duo1p was localized by immunofluorescence, by immunoelectron microscopy, and by tagging with green fluorescent protein (GFP), to intranuclear spindle microtubules and spindle pole bodies. Temperature-sensitive duo1 mutants arrest with short spindles. This arrest is dependent on the mitotic checkpoint. Dam1p was identified by two-hybrid analysis as a protein that binds to Duo1p. By expressing a GFP–Dam1p fusion protein in yeast, Dam1p was also shown to be associated with intranuclear spindle microtubules and spindle pole bodies in vivo. As with Duo1p, overproduction of Dam1p caused mitotic defects. Biochemical experiments demonstrated that Dam1p binds directly to microtubules with micromolar affinity. We suggest that Dam1p might localize Duo1p to intranuclear microtubules and spindle pole bodies to provide a previously unrecognized function (or functions) required for mitosis.
Related Links	http://jcb.rupress.org/content/143/4/1029.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2132964/pdf http://jcb.rupress.org/content/jcb/143/4/1029.full.pdf
Ending Page	1040
Page Count	12
Starting Page	1029
DOI	10.1083/jcb.143.4.1029
Journal	Journal of Cell Biology
Issue Number	4
Volume Number	143
Language	English
Publisher	Rockefeller University Press
Publisher Date	1998-11-16
Access Restriction	Open
Subject Keyword	Genetics Mitosis Microtubule Anaphase Cytoskeleton Spindle Journal: Journal of Cell Biology (Vol- 143, Issue- 4)
Content Type	Text

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