Loading...
Please wait, while we are loading the content...
Similar Documents
Simultaneous determination three phytosterol compounds, campesterol, stigmasterol and daucosterol in Artemisia apiacea by high performance liquid chromatography-diode array ultraviolet/visible detector
| Content Provider | Scilit |
|---|---|
| Author | Ma, Choong Je Lee, Jiwoo Weon, Jin Bae Yun, Bo-Ra Eom, Min Rye |
| Copyright Year | 2015 |
| Abstract | Background: Artemisia apiacea is a traditional herbal medicine using treatment of eczema and jaundice in Eastern Asia, including China, Korea, and Japan. Objective: An accurate and sensitive analysis method using high performance liquid chromatography-diode array ultraviolet/visible detector and liquid chromatography-mass spectrometry for the simultaneous determination of three phytosterol compounds, campesterol, stigmasterol and daucosterol in A. apiacea was established. Materials and Methods: The analytes were separated on a Shiseido C$ _{18}$ column (5 μm, 4.6 mm I.D. ×250 mm) with gradient elution of 0.1% trifluoroacetic acid and acetonitrile. The flow rate was 1 mL/min and detection wavelengths were set at 205 and 254 nm. Results: Validation of the method was performed to demonstrate its linearity, precision and accuracy. The calibration curves showed good linearity $(R^{2}$ > 0.9994). The limits of detection and limits of quantification were within the ranges 0.55-7.07 μg/mL and 1.67-21.44 μg/mL, respectively. And, the relative standard deviations of intra- and inter-day precision were <2.93%. The recoveries were found to be in the range of 90.03-104.91%. Conclusion: The developed method has been successfully applied to the analysis for quality control of campesterol, stigmasterol and daucosterol in A. apiacea. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378127/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=42;spage=297;epage=303;aulast=Lee;type=2 |
| Ending Page | 303 |
| Page Count | 7 |
| Starting Page | 297 |
| File Format | XHTML |
| ISSN | 09731296 |
| e-ISSN | 09764062 |
| DOI | 10.4103/0973-1296.153082 |
| Journal | Pharmacognosy Magazine |
| Issue Number | 42 |
| Volume Number | 11 |
| Language | English |
| Publisher | Medknow |
| Publisher Date | 2015-01-01 |
| Access Restriction | Open |
| Subject Keyword | Integrative and Complementary Medicine Artemisia Apiacea High Performance Liquid Chromatography-diode Array Ultraviolet/visible Detector Liquid Chromatography-mass Spectrometry Simultaneous Determination Pharmacognosy Magazine, Volume 11, Issue 42 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
