NDLI: Simultaneous determination of four bioactive compounds in Verbena officinalis L. by using high-performance liquid chromatography

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Simultaneous determination of four bioactive compounds in Verbena officinalis L. by using high-performance liquid chromatography

Content Provider	Scilit
Author	Cao, Gang Cong, Xiao-Dong Zhang, Yun Cai, Bao-Chang Liu, Zhen Xu, Zhiwei Zhou, Hanyu
Copyright Year	2012
Abstract	Background: Verbena officinalis L., called mabiancao in Chinese, is derived from the aerial part of Herba Verbanae. It is a traditional Chinese medicine commonly used in China and northern Europe, which is widely used for clearing away heat and detoxicating, promoting blood circulation, and removing blood stasis. This paper describes a sensitive and specific assay for the determination of four bioactive compounds in V. officinalis L. Materials and Methods: In this paper, the four components were separated on an Agilent Zorbax Extend C$ _{18}$ column (250 mm × 4.6 mm × 5 μm) and detected by a diode array detector. The mobile phase was composed of (a) aqueous phosphoric acid (0.1%, v/v) and (b) acetonitrile using a gradient elution. Analytes were performed at 30°C with a flow rate of 1.0 ml/min and UV detection at 203, 238, and 331 nm. Results: All calibration curves showed good linear regression $(r^{2}$ ≥ 0.9999) within tested ranges. Overall intra- and interday variations were less than 1.84%, and the average recoveries were 97.32-102.81% for analytes. Discussion and Conclusion: The proposed method would be sensitive enough and reliable for comprehensive quality control for clinical use and modernization of V. officinalis L.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371439/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2012;volume=8;issue=30;spage=162;epage=165;aulast=Liu;type=2
File Format	XHTML
ISSN	09731296
e-ISSN	09764062
DOI	10.4103/0973-1296.96575
Journal	Pharmacognosy Magazine
Issue Number	30
Volume Number	8
Language	English
Publisher	Medknow
Access Restriction	Open
Subject Keyword	Integrative and Complementary Medicine High-performance Liquid Chromatography-diode Array Detection Quality Control Simultaneous Determination Verbena Officinalis L. Pharmacognosy Magazine, Volume 8, Issue 30
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Pharmaceutical Science

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