NDLI: Qualitative and quantitative analysis of nine major compounds in the Bozhougyiqi-Tang using a high-performance liquid chromatography coupled with a diode array detector and electrospray ionization mass spectrometer

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Qualitative and quantitative analysis of nine major compounds in the Bozhougyiqi-Tang using a high-performance liquid chromatography coupled with a diode array detector and electrospray ionization mass spectrometer

Content Provider	Scilit
Author	Ma, Choong Je Weon, Jin Bae Yang, Hye Jin Yun, Bo-Ra Ma, Jin Yeul Lee, Bohyoung
Copyright Year	2013
Abstract	Background: Bozhougyiqi-Tang (BZYQT) is of traditional herbal medicine used for enhancement of digestive capacity. Objective: An accurate and reliable simultaneous determination using a HPLC-DAD and ESI-MS was developed and validated for the qualitative and quantitative analysis of 9 major compounds, ferulic acid (1), naringin (2), hesperidin (3), decursinol (4), glycyrrhizin (5), saikosaponin A (6), 6-gingerol (7), ginsenoside Rg3 (8), decursin (9), in traditional herbal medicine 'Bozhougyiqi-Tang.' Materials and Methods: The chromatographic separation of 9 compounds was performed on a SHISEIDO C$ _{18}$ column (250 mm × 4.6 mm i.d., S-5 μm) using gradient elution with 0.1% trifluoroacetic acid and acetonitrile at a flow rate of 1.0 ml/min. The 9 compounds were identified based on peak retention time and UV spectrum and MS data of these compounds. Results: This developed method showed good linearity (R$ ^{2}$ > 0.999). The LOD and LOQ of the major compounds were less than 0.09 and 0.28 μg/ml, respectively. The intra - day and inter - day RSD values were within 2.06% and 1.64%, respectively. The mean recoveries were from 92.10% to 108.56% with less than 1.88%. The results indicated that established method had good precision and accuracy. Conclusion: The new method was successfully applied to the simultaneous analysis of 9 compounds in Bozhougyiqi-Tangs samples.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732432/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2013;volume=9;issue=35;spage=271;epage=282;aulast=Weon;type=2
File Format	XHTML
ISSN	09731296
e-ISSN	09764062
DOI	10.4103/0973-1296.113291
Journal	Pharmacognosy Magazine
Issue Number	35
Volume Number	9
Language	English
Publisher	Medknow
Access Restriction	Open
Subject Keyword	Integrative and Complementary Medicine Bozhougyiqi-tang Simultaneous Determination High-performance Liquid Chromatography-diode Array Detector Liquid Chromatography-mass Spectrometry Major Compounds Pharmacognosy Magazine, Volume 9, Issue 35
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Pharmaceutical Science

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