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Phytochemical analysis on quantification and the inhibitory effects on inflammatory responses from the fruit of xanthii fructus
| Content Provider | Scilit |
|---|---|
| Author | Jeong, Soo-Jin Yoo, Sae-Rom Seo, Chang-Seob Lee, Na-Ri Shin, Hyeun-Kyoo |
| Copyright Year | 2015 |
| Abstract | Objective: Xanthii fructus (Compositae) is a traditional herbal medicine used for treating headache, toothache, pruritus, empyema, and rhinitis. In this study of the quality control of X. fructus, we performed simultaneous analysis of nine marker compounds: Protocatechuic acid (1), chlorogenic acid (2), caffeic acid (3), 4,5-dicaffeoylquinic acid (4), ferulic acid (5), 3,5-dicaffeoylquinic acid (6), 1,3-dicaffeoylquinic acid (7), 1,4-dicaffeoylquinic acid (8), and 4,5-dicaffeoylquinic acid (9). Materials and Methods: Nine components were separated using reversed-phase SunFire™ C$ _{18}$ analytical column and analyzed using high-performance liquid chromatography. We examined the biological effects of the nine marker compounds by determining their anti-inflammatory activities in the murine macrophage cell line RAW 264.7. Results: Among the nine marker compounds, eight significantly inhibited lipopolysaccharide (LPS)-stimulated tumor necrosis factor-alpha (TNF-a) production. 1, 3, 5 had significant inhibitory effects on LPS-induced prostaglandin E$ _{2}$ (PGE$ _{2}$ ) production in RAW 264.7 cells. None of the tested marker compounds had a significant effect on interleukin-6 production in LPS-treated RAW 264.7 cells. Our data demonstrated that each marker compound from X. fructus exerts anti-inflammatory activity by targeting different inflammation-related pathways such as the TNF-a or PGE$ _{2}$ pathway. Conclusion: Further experiments using in vitro and in vivo models are needed to identify the mechanisms responsible for the anti-inflammatory properties of each marker compound. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4787093/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2015;volume=11;issue=44;spage=585;epage=591;aulast=Yoo;type=2 |
| File Format | XHTML |
| ISSN | 09731296 |
| e-ISSN | 09764062 |
| DOI | 10.4103/0973-1296.172966 |
| Journal | Pharmacognosy Magazine |
| Issue Number | 44 |
| Volume Number | 11 |
| Language | English |
| Publisher | Medknow |
| Access Restriction | Open |
| Subject Keyword | Integrative and Complementary Medicine Anti-inflammatory Effect Raw 264.7 Xanthii Fructus Herbal Medicine High-performance Liquid Chromatography Pharmacognosy Magazine, Volume 11, Issue 44 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
