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RNAi-mediated inhibition of Raf-1 leads to decreased angiogenesis and tumor growth in gastric cancer
| Content Provider | Scilit |
|---|---|
| Author | Meng, Fanping Dong, Baoxia Li, Hanwei Fan, Daiming Ding, Jie |
| Copyright Year | 2009 |
| Description | Journal: Cancer Biology & Therapy Tumor angiogenesis plays an important role in the malignancy of solid tumors. A number of recent studies including our own have suggested that Raf-1 is involved in this process, and may be critical in regulating gene activation of several angiogenesis factors. RNA interference (RNAi) provides a powerful method for gene silencing in eukaryotic cells, including proliferating mammalian cells. To further define Raf-1 function in angiogenesis and to explore novel approaches to modulate angiogenesis, we employed the small interference RNA technique to knockdown gene expression of Raf-1 in gastric cancer cells and observed the effect of silencing Raf-1 on gastric cancer tumorigenesis and angiogenesis in vitro and in nude mice. We found that the expression of double stranded RNA leads to the efficient and specific inhibition of endogenous Raf-1 protein expression in gastric cancer cell lines as determined by Western blotting. Raf-1 protein is a potential target for gastric cancer biological therapy. Inhibition of Raf-1 with siRNA technique could inhibit growth of the tumor graft and reduce angiogenesis in nude mice, which probably caused by down regulation of pro-angiogenesis molecules, such as VEGF and HIF-1α.Taken together, our findings indicate that specific Raf-1 targeting may have important therapeutic values for cancer therapy, and that individual Raf-1 may be a useful target in developing angiogenic inhibitors. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.4161/cbt.8.2.7208?needAccess=true |
| Ending Page | 179 |
| Page Count | 6 |
| Starting Page | 174 |
| ISSN | 15384047 |
| e-ISSN | 15558576 |
| DOI | 10.4161/cbt.8.2.7208 |
| Journal | Cancer Biology & Therapy |
| Issue Number | 2 |
| Volume Number | 8 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2009-01-15 |
| Access Restriction | Open |
| Subject Keyword | Journal: Cancer Biology & Therapy Biochemistry and Molecular Biology Gene Expression Angiogenesis Gastric Cancer Tumor Growth Inhibition of Raf |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Pharmacology Molecular Medicine Oncology |
