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Inhibition of gastric cancer angiogenesis by vector-based RNA interference for Raf-1
| Content Provider | Scilit |
|---|---|
| Author | Meng, Fanping Ding, Jie Liu, Na Zhang, Jing Shao, Xiaodong Shen, Huiqin Xue, Yan Xie, Huahong Fan, Daiming |
| Copyright Year | 2005 |
| Description | Journal: Cancer Biology & Therapy Tumor angiogenesis plays an important role in the malignancy of solid tumors. A number of recent studies including our own have suggested that Raf-1 is involved in this process, and may be critical in regulating gene activation of several angiogenesis factors. To further define Raf-1 function in angiogenesis and to explore novel approaches to modulate angiogenesis, we employed the small interference RNA technique to knockdown gene expression of Raf-1 in gastric cancer cell line SGC7901 that expresses a high level of Raf-1. The protein level of Raf-1 in the SGC7901cells was decreased dramatically after transfection with a Raf-1 specific siRNA vector. Further study proved that, VEGF and HIF-1£\, two angiogenesis promoting factors, were found to be downregulated. And we also find that Vector-based RNA interference for Raf-1 increases transfected gastric cell apoptosis and inhibits cellular proliferation. Our results suggest that Raf-1 may be involved in angiogenesis by controlling the expression of angiogenesis-related factors and provide a possible strategy for the treatment of tumor angiogenesis by targeting Raf-1. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.4161/cbt.4.1.1446?needAccess=true |
| Ending Page | 124 |
| Page Count | 5 |
| Starting Page | 120 |
| ISSN | 15384047 |
| e-ISSN | 15558576 |
| DOI | 10.4161/cbt.4.1.1446 |
| Journal | Cancer Biology & Therapy |
| Issue Number | 1 |
| Volume Number | 4 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2005-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Cancer Biology & Therapy Biochemistry and Molecular Biology Gene Expression Angiogenesis Suggested That Raf Vector Based Interference for Raf Based Rna Interference |
| Content Type | Text |
| Subject | Cancer Research Pharmacology Molecular Medicine Oncology |
