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Haemoglobin (Erythrogen™)-Ehhanced Post-Thaw Growth of Cryopreserved Cells
| Content Provider | Scilit |
|---|---|
| Author | Azhakanandam, K. Anthony, P. Lowe, K. C. Davey, M. R. Power, J. B. |
| Copyright Year | 1999 |
| Description | Journal: Artificial cells, blood substitutes, and immobilization biotechnology Supplementation of semi-solid R2 culture medium with a commercial bovine haemoglobin (Hb) solution (Erythrogen™) at 1:50-1:500 (v:v), had beneficial effects on the growth, following cryopreservation, of cells of the Indica rice, Oryza sativa cv. Pusa Basmati 1. The mean absorbance, as assessed by triphenyl tetrazolium chloride reduction, of rice cells at 8 d post-thawing, was increased by up to 60% (P < 0.05), compared to cells recovered in the absence of Hb. Eryihrogen™ (1:50-1:500 v:v) promoted an increase in biomass, of up to 25% over control (P < 0.05), at 24 d post-thawing. Cell suspensions, re-established by transfer to liquid medium of cells initially thawed and cultured with Erythrogen™ for 24 d, exhibited increased (up to 2-fold) growth rates over a subsequent 20-d period, compared to cells recovered without Hb. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.3109/10731199909117690 |
| Ending Page | 169 |
| Page Count | 7 |
| Starting Page | 163 |
| ISSN | 10731199 |
| e-ISSN | 15324184 |
| DOI | 10.3109/10731199909117690 |
| Journal | Artificial cells, blood substitutes, and immobilization biotechnology |
| Issue Number | 2 |
| Volume Number | 27 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1999-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Artificial cells, blood substitutes, and immobilization biotechnology Agriculture and Animal Production Cryopreserved Cells Ehhanced Post |
| Content Type | Text |
| Subject | Biomedical Engineering Biotechnology |
