Loading...
Please wait, while we are loading the content...
Similar Documents
Haemoglobin-Enhanced Mitotic Division in Cultured Protoplasts
| Content Provider | Scilit |
|---|---|
| Author | Anthony, P. Lowe, K. C. Davcy, M. R. Power, J. B. |
| Copyright Year | 1997 |
| Description | Protoplasts from cell suspensions of albino Petunia hybrida cv. Comanche were cultured for 9 days in nutrient medium containing Erythrogen™, a purified bovine haemoglobin solution (supplied at 10% w/v) at 1:50 - 1:500 (v/v). In some assessments, the non-ionic surfactant Pturonic® F-68 (Poloxamcr 188), was also added to the culture medium at 0.01-1.0% (w/v). Erythrogen™ at 1:50 (v/v) increased the mean initial protoplast plating efficiency (IPE; 18.5 + 0.8%, n = 5 throughout) by 64% (P < 0.001) above that of controls (11.3 ± 0.4%). Supplementation of medium with 1:50 (v:v) Erythrogen™ and 0.01% (w/v) Pluronic® F-68, increased the mean IPE (24.4 ± 1.4%) by 92% (P < 0.001) over control (12.7 ± 1.1%). Similar results were obtained for mesophyll protoplasts of Passiflora suberosa, with 1:50 and 1:100 (v/v) Erythrogen™ increasing the mean IPEs to 87% and 93% respectively, over controls. This beneficial and synergistic effect of Erythrogen™ with Pluronic® F-68, on mitotic division of cultured Petunia and Passiflora protoplasts, should also facilitate the culture of isolated protoplasts and cells of other, agronomically-important, species. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.3109/10731199709117454 |
| Ending Page | 583 |
| Page Count | 7 |
| Starting Page | 577 |
| ISSN | 10731199 |
| e-ISSN | 15324184 |
| DOI | 10.3109/10731199709117454 |
| Journal | Artificial cells, blood substitutes, and immobilization biotechnology |
| Issue Number | 6 |
| Volume Number | 25 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1997-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology Horticulture Haemoglobin Medium Division Petunia Protoplasts W/v V/v Mitotic Ipe Erythrogen |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biomedical Engineering Biotechnology |
