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Enhanced Post-Thaw Viability of Cryopreserved Cells by Oxygenated Perfluorocarbon orPluronic® F-68
| Content Provider | Scilit |
|---|---|
| Author | Anthony, P. Lowe, K. C. Davey, M. R. Power, J. B. |
| Copyright Year | 1998 |
| Description | Viability and growth following cryopreservation have been examined following (i) culture of Oryza sativa cv. Taipei 309 (Japonica rice) in medium overlaying oxygenated perfluorocarbon (PFC) liquid (Flutec® PP6), and (ii) culture of two cvs. of Japonica rice (Taipei 309, Tarom), and Lolium multiflorum in media with Pluronic® F-68 (0.01–0.2% w/v). The mean viability of cv. Taipei 309 rice cells following 4 days in medium overlaying oxygenated Flutec® (0.45 ± 0.07; n = 20) was significantly (P < 0.05) greater than control (0.35 ± 0.08). Cell viability of cv. Tarom was increased by 400% (P < 0.001) with 0.1% (w/v) Pluronic® F-68 in the recovery medium. Mean cell viability was also elevated 3-fold (P < 0.001) and 2-fold (P < 0.05) with 0.2% (w/v) and 0.01% (w/v) Pluronic, respectively. A 2-fold increase (P < 0.001) in viability occurred for Taipei 309 cells exposed to 0.01% (w/v) Pluronic® F-68; that of Lolium cells was increased by 31% (P < 0.01) under similar conditions. Pluronic® F-68 (0.01% w/v) also stimulated biomass, by 29–32%, in both cvs. Taipei 309 and Lolium at 30 days post-thawing. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.3109/10731199809118944 |
| Ending Page | 33 |
| Page Count | 7 |
| Starting Page | 27 |
| ISSN | 10731199 |
| e-ISSN | 15324184 |
| DOI | 10.3109/10731199809118944 |
| Journal | Artificial cells, blood substitutes, and immobilization biotechnology |
| Issue Number | 1 |
| Volume Number | 26 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1998-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology Food Science and Technology Viability Pluronic Perfluorocarbon Cryopreserved Oxygenated Thawing Japonica Fold Pfc Pp6 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biomedical Engineering Biotechnology |
