NDLI: Silencing of TKTL1 by siRNA inhibits proliferation of human gastric cancer cells in vitro and in vivo

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Silencing of TKTL1 by siRNA inhibits proliferation of human gastric cancer cells in vitro and in vivo

Content Provider	Scilit
Author	Yuan, Weijie Wu, Shaobin Guo, Jianwu Chen, Zhikang Ge, Jie Yang, Pu Hu, Bin Chen, Zihua
Copyright Year	2010
Description	Tumorigenesis requires energy production via aerobic glycolysis (the Warburg effect) in malignant tumors. Recent research has demonstrated that the pentose phosphate pathway (PPP) is augmented in some tumors, especially the non-oxidative part of the PPP which is controlled by transketolase (TKT) enzyme reactions. One TKT isoform, transketolase-like protein 1 (TKTL1), is specifically up-regulated in different human cancers, and its over-expression predicts poor patient survival. To further define the function of in malignant progression, we employed the small interference RNA (siRNA) technique to knockdown gene expression of TKTL1 in the gastric cancer cell line AGS. We used TKTL1 siRNA to observe the effect of reduced TKTL1 expression on gastric cancer tumorigenesis in a nude mice xenograft model and on proliferation in vitro. Our results showed that the expression of double stranded RNA led to the efficient and specific inhibition of endogenous TKTL1 expression in AGS cells. In addition, the TKT activity was significantly deceased in the TKTL1 siRNA-treated AGS cells. TKTL1 suppression resulted in delayed cell proliferation in vitro. Furthermore, loss of TKTL1 inhibited the growth of AGS tumor xenografts. Altogether, our findings indicate that the specific inhibition of TKTL1 may be important therapeutically.
Related Links	https://www.tandfonline.com/doi/pdf/10.4161/cbt.9.9.11431?needAccess=true
Ending Page	716
Page Count	7
Starting Page	710
ISSN	15384047
e-ISSN	15558576
DOI	10.4161/cbt.9.9.11431
Journal	Cancer Biology & Therapy
Issue Number	9
Volume Number	9
Language	English
Publisher	Informa UK Limited
Publisher Date	2010-05-01
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Gene Expression Proliferation Protein Function Survival Tktl1 Sirna
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pharmacology Molecular Medicine Oncology

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