NDLI: Silencing stathmin gene expression by survivin promoter-driven siRNA vector to reverse malignant phenotype of tumor cells

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Silencing stathmin gene expression by survivin promoter-driven siRNA vector to reverse malignant phenotype of tumor cells

Content Provider	Scilit
Author	Zhang, Hui-Zhong Wang, Yan Gao, Ping Lin, Fang Liu, Li Yu, Bing Ren, Ji-Hong Zhao, Hui Wang, Rui
Copyright Year	2006
Description	Stathmin gene over-expression has been shown to play an important role in maintenance of malignant phenotype in tumor cells, and the blocking efficacy and tumor specificity of this target has been concerned in clinical trails. In this report, we designed survivin promoter-driven siRNA eukaryotic expression vector that expressed the small interfering RNA targeting stathmin gene to selectively knock down the stathmin gene expression in two different kinds of tumor cell lines while sparing normal cell lines. The therapeutic potential of this recombinant vector was tested in human cervical cancer Hela cells and osteosarcoma SSOP-9607 cells, and in human umbilical vein endothelial cell line ECV304 cells as control. The siRNA vector- transfected Hela cells and SSOP-9607 cells revealed marked inhibition of stathmin expression and a dramatic growth inhibition comparing with ECV304 cells, parental-vector transfected cells and untransfected cells.Cell cycle analysis of siRNA vector transfected tumor cells by Flow Cytometry showed G2/M phase block, while morphologic analysis by TURNEL staining method showed marked increase of apoptosis. Our study indicates that survivin gene promoter-driven stathmin siRNA expression vector may have potential use in tumor gene therapy with targeted tumor gene silencing effect.
Related Links	https://www.tandfonline.com/doi/pdf/10.4161/cbt.5.11.3272?needAccess=true
Ending Page	1461
Page Count	5
Starting Page	1457
ISSN	15384047
e-ISSN	15558576
DOI	10.4161/cbt.5.11.3272
Journal	Cancer Biology & Therapy
Issue Number	11
Volume Number	5
Language	English
Publisher	Informa UK Limited
Publisher Date	2006-11-14
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Gene Expression Small Interfering Rna Gene Therapy Efficacy Tumor Cells Sirna Vector Blocking Silencing Stathmin
Content Type	Text
Subject	Cancer Research Pharmacology Molecular Medicine Oncology

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