Loading...
Please wait, while we are loading the content...
Similar Documents
Effects of a Novel Perfluorocarbon Emulsion on Neutrophil Chemiluminescence in Human Whole Blood in Vitro
| Content Provider | Scilit |
|---|---|
| Author | Edwards, C. M. Lowe, K. C. Röhlke, W. Geister, U. Reuter, P. Meinert, H. |
| Copyright Year | 1997 |
| Description | The effects have been studied of a novel perfluorochemical (PFC) emulsion (18.5% perfluorodecalin, 1.5% perfluorodimorpholine propane, 2.5% lecithin) on phorbol 12-myristate 13-acetate (PMA; 100 μg $ml^{−1}$)-induced neutrophil chemiluminescence in citrated human whole blood in vitro. A transient, dose-dependent, decrease in chemiluminescence, to a maximum of 54% after 12 min (P < 0.05), occurred when blood was pre-incubated with 10–40 μl of the PFC emulsion, compared to saline controls. The mean (± s.e.m., n = 6) chemiluminescence of neutrophils incubated with 30 μl emulsion at 12 min following PMA stimulation (9.5 ± 1.3 mV) was significantly lower (P < 0.05) than control (24.2 ± 2.2 mV). Incubation of blood with lecithin up to 16 mg $ml^{−1}$ and Pluronic® F-68 or Pluronic® PE 6800 up to 65 mg $ml^{−1}$ did not affect chemiluminescence. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.3109/10731199709118915 |
| Ending Page | 260 |
| Page Count | 6 |
| Starting Page | 255 |
| ISSN | 10731199 |
| e-ISSN | 15324184 |
| DOI | 10.3109/10731199709118915 |
| Journal | Artificial cells, blood substitutes, and immobilization biotechnology |
| Issue Number | 3 |
| Volume Number | 25 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1997-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology Anesthesiology Emulsion Neutrophil Chemiluminescence Pluronic Transient S.e.m |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biomedical Engineering Biotechnology |
