NDLI: Measurement of Telomere Length

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Measurement of Telomere Length

Content Provider	Scilit
Author	Pejenaute, Álvaro Goñi, Guillermo Zalba
Copyright Year	2017
Description	This chapter reviews the main methods in telomere length measurement comparing the technologies and pointing their strong points and weaknesses. These methodologies can be classified in four groups: terminal restriction fragment analysis (TRF), polymerase chain reaction (PCR) based methods, quantitative fluorescence in situ hybridization methods (Q-FISH), and single telomere length analysis (STELA) technology. The chapter describes the two less used methods: Hybridization protection assay (HPA) and Primed in situ (PRINS). TRF analysis was the first technique used to measure average telomere length, and since it was for many years the only method available it is described as the "gold standard" in telomere quantification. Flow FISH methodology combines the use of cell sorting by flow cytometry with the basis of FISH applied to telomere length measurement. STELA analysis was developed to solve the limitations of TRF and Q-FISH and initially use to study the short arms of sex chromosomes. Book Name: Telomeres, Diet and Human Disease
Related Links	https://api.taylorfrancis.com/content/chapters/edit/download?identifierName=doi&identifierValue=10.1201/9781315152431-3&type=chapterpdf
Ending Page	54
Page Count	16
Starting Page	39
DOI	10.1201/9781315152431-3
Language	English
Publisher	Informa UK Limited
Publisher Date	2017-08-30
Access Restriction	Open
Subject Keyword	Book Name: Telomeres, Diet and Human Disease Medical Informatics Trf Flow Hybridization Fish Telomere Length Measurement Stela
Content Type	Text
Resource Type	Chapter

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