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Effect of Plasminogen Activator Inhibitor-1 on Tissue-Type Plasminogen Activator-Induced Fibrinolysis
| Content Provider | Scilit |
|---|---|
| Author | Carr, Marcus E. Krishnamurti, C. |
| Editor | Alving, B. M. |
| Copyright Year | 1992 |
| Description | The effect of fibrin on the interaction of human recombinant single-chain tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) was studied in normal rabbit plasma and in plasma with high levels of native PAI-1. t-PA was added to diluted plasma containing calcium (10 mM) and$ ^{125}$I-fibrinogen at 37° C. Clotting was initiated with human thrombin, and lysis was monitored both turbidimetrically and by release of$ ^{125}$I-fibrin degradation products (fdp). The activity of t-PA (50 IU/ml) was rapidly reduced to 15% of the initial value in plasma containing PAI-1 (23 AU/ml). When thrombin and t-PA were added simultaneously to the plasma, more than 70% of the activity was retained through incorporation of t-PA into the fibrin clot. t-PA-induced fibrinolysis in PAI-1 enriched plasma was further delayed when the temperature was reduced from 37 to 25° C. Turbidimet-ric and$ ^{125}$I-fdp release data provided complementary information. The former technique traced fiber dissolution, while the latter reflected network integrity. These results indicate that t-PA-induced fibrinolysis in PAI-1 enriched plasma is modulated by the presence of fibrin and by temperature. |
| Related Links | http://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-0038-1648389.pdf |
| Ending Page | 110 |
| Page Count | 5 |
| Starting Page | 106 |
| ISSN | 2567689X |
| DOI | 10.1055/s-0038-1648389 |
| Journal | Thrombosis and Haemostasis |
| Issue Number | 01 |
| Volume Number | 67 |
| Language | English |
| Publisher | Georg Thieme Verlag KG |
| Publisher Date | 1992-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Thrombosis and Haemostasis Induced Fibrinolysis Plasminogen Activator |
| Content Type | Text |
| Resource Type | Article |
| Subject | Hematology |
