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Plasminogen Activator Inhibitor-1 Is the Primary Inhibitor of Tissue-Type Plasminogen Activator in Pregnancy Plasma
| Content Provider | Scilit |
|---|---|
| Author | Jørgensen, Maja Philips, Malou Thorsen, Sixtus Selmer, Johan Zeuthen, Jesper |
| Copyright Year | 1987 |
| Description | The aim of the present work was to clarify to what extent plasminogen activator inhibitor-1 (PAI-1) and plasminogen activator inhibitor-2 (PAI-2) contribute to the increase in plasma inhibition of tissue-type plasminogen activator (t-PA) observed during pregnancy. It was demonstrated that a monoclonal antibody against PAI-1 almost completely quenched inhibition of single-chain t-PA and most of the inhibition of two-chain t-PA in plasma during the third trimester of piegnancy. The remaining inhibition of two-chain t-PA was to a great extent abolished by a PAI-2 antibody. The second order rate constant $(k_{1}$) for inhibition of single-chain t-PA by the inhibitor neutralized by the PAI-1 antibody was about 4.8 · $10^{6}$ $M^{-1}$ · $s^{-1}$. The conversion of singlechain t-PA to the two-ehain form increased the reaction rate with the inhibitor about 3-fold. These kinetic data are compaiable with those obtained with TAI-l in non-pregnancy plasma oi with purified PAI-1. From the above results it is concluded that PAI-1 is the primary inhibitor of both single-chain and two chain t PA and that PAI-2 is the secondary inhibitor of two-chain t-PA in pregnancy plasma. The concentration of reactive PAI-1 versus gestation age was assayed in plasma from 6 women by binding of PAI-1 to$ ^{125}$I-labelled single-chain t-PA followed by quantitation of the labelled t-PA-PAI-1 complex after separation by SDS- polyacrylamide gel electrophoresis. It was found that the concentration of PAI-1 increased 4 to 8-fold during the gestation period reaching a level of about 1.4 nM at term. Post partum the plasma concentration declined abruptly within 24 h to the level observed in age-matched non-pregnant women. |
| Related Links | http://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-0038-1646006.pdf |
| Ending Page | 878 |
| Page Count | 7 |
| Starting Page | 872 |
| ISSN | 2567689X |
| DOI | 10.1055/s-0038-1646006 |
| Journal | Thrombosis and Haemostasis |
| Issue Number | 03 |
| Volume Number | 58 |
| Language | English |
| Publisher | Georg Thieme Verlag KG |
| Publisher Date | 1987-10-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Thrombosis and Haemostasis Biochemical Research Plasminogen Activator Inhibitor-1 Plasminogen Activator Inhibitor-2 Tissue-type Plasminogen Activator |
| Content Type | Text |
| Resource Type | Article |
| Subject | Hematology |
