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In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract
| Content Provider | Scilit |
|---|---|
| Author | Daltro, Sérgio Ricardo Teixeira Santos, Ivanilson Pimenta Barros, Paula Ladeia Moreira, Diogo Rodrigo Magalhães Tomassini, Therezinha Coelho Barbosa Ribeiro, Ivone Maria Santos, Ricardo Ribeiro dos Meira, Cássio Santana Soares, Milena Botelho Pereira |
| Copyright Year | 2020 |
| Description | The need for new immunomodulatory drugs is due to the side effects associated with the prolonged use of the currently used immunomodulatory drugs. In this context, the present work aimed to investigate the immunomodulatory effect of an ethanolic concentrated extract from Physalis angulata. The cytotoxicity of samples was determined using peritoneal macrophages though the Alamar Blue assay. The immunomodulatory activity of the ethanolic extract from P. angulata on activated macrophages was determined by measurement of nitrite and cytokine production. The immunosuppressive effects of the ethanolic extract from P. angulata was evaluated on lymphocyte proliferation and cytokine production. The effects of the extract on cell cycle progression and cell death on lymphocytes were evaluated by flow cytometry. Lastly, the ethanolic extract from P. angulata was tested in vivo in toxicological tests and in models of peritonitis and delayed-type hypersensitivity response. The ethanolic extract from P. angulata decreased nitrite, interleukin-6, interleukin-12, and TNF-α production by activated macrophages without affecting the cell viability. In addition, the ethanolic extract from P. angulata inhibited lymphoproliferation and the secretion of interleukin-2, interleukin-6, and IFN-γ, and increased interleukin-4 secretion by activated splenocytes. Flow cytometry analysis in lymphocyte cultures showed that treatment with the ethanolic extract from P. angulata induces cell cycle arrest in the G1 phase followed by cell death by apoptosis. Moreover, mice treated with the extract from P. angulata at 100 or 200 mg/kg did not show signs of toxicity or alterations in serum components. Finally, the ethanolic extract from P. angulata significantly reduced neutrophil migration and reduced paw edema in bovine serum albumin-induced the delayed-type hypersensitivity response model. Our results demonstrate the potential of the ethanolic extract of P. angulata as an alternative for the treatment of immune-inflammatory diseases. |
| Related Links | http://www.thieme-connect.de/products/ejournals/pdf/10.1055/a-1237-4268.pdf |
| Ending Page | 168 |
| Page Count | 9 |
| Starting Page | 160 |
| ISSN | 00320943 |
| e-ISSN | 14390221 |
| DOI | 10.1055/a-1237-4268 |
| Journal | Planta Medica |
| Issue Number | 01/02 |
| Volume Number | 87 |
| Language | English |
| Publisher | Georg Thieme Verlag KG |
| Publisher Date | 2020-09-16 |
| Access Restriction | Open |
| Subject Keyword | Journal: Planta Medica Integrative and Complementary Medicine Lymphocyte Function Physalis Angulata Macrophage Activation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Drug Discovery Analytical Chemistry Molecular Medicine Pharmacology Complementary and Alternative Medicine Pharmaceutical Science |
