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Protein kinase C regulates proliferation of mast cells and the expression of the mRNAs of fos and jun proto-oncogenes during activation by IgE-Ag or calcium ionophore A23187
| Content Provider | Scilit |
|---|---|
| Author | Baranes, D. Razin, E. |
| Copyright Year | 1991 |
| Abstract | Short-term stimulation (up to 16 hours) of interleukin-3 (IL-3)- dependent mouse bone marrow-derived mast cells, Abelson transformed mouse liver-derived mast cells, or rat basophilic leukemia cells by either IgE-Ag or calcium ionophore A23187 resulted in inhibition of their proliferation as measured by 3H-thymidine incorporation and MTT (tetrazolium) assays, and in accumulation of the mRNAs of c-fos, c-jun, junB and slightly of junD proto-oncogenes. The involvement of protein kinase C (PKC) in these responses was investigated by using several approaches of enzyme activity regulation. Direct activation of the PKC was achieved by short-term exposure of the cells to the PKC-specific activator phorbol 12-myristate-13-acetate (PMA). Inhibition of PKC activity was obtained by either prolonged treatment of the cells with PMA or by exposure of the cells to the PKC inhibitors H-7 and staurosporine. The results showed the following: (1) Short-term exposure of mast cells to PMA was sufficient to induce inhibition of proliferation. (2) An increase in PKC activity was associated with a decrease in the proliferation of IgE-dinitrophenol (DNP) or calcium ionophore A23187-stimulated cells. (3) A direct correlation was found between the increase in PKC activity and the increase in the level of the mRNAs of the jun proto-oncogenes in cells activated by both stimuli mentioned. (4) While an increase in PKC activity was associated with the upregulation of the level of c-fos mRNA during calcium ionophore A23187 stimulation, it showed the opposite effect on the expression of the mRNA of this proto-oncogene when the cells were triggered by IgE- DNP. Therefore, we concluded that PKC plays various roles in the expression of the mRNA of c-fos in activated mast cells depending on the stimulus involved. In addition, the expression of the mRNA of c-jun and junB proto-onogenes is not coordinately regulated with that of c- fos during immunologic stimulation. This discordancy, which is associated with the increase in PKC activity in mast cells, may play a role in the regulation of the transcription of AP-1-responsive genes, and therefore could be associated with the regulation of proliferation of these cells. |
| Related Links | https://ashpublications.org/blood/article-pdf/78/9/2354/224607/2354.pdf |
| Ending Page | 2364 |
| Page Count | 11 |
| Starting Page | 2354 |
| DOI | 10.1182/blood.v78.9.2354.bloodjournal7892354 |
| Journal | Blood |
| Issue Number | 9 |
| Volume Number | 78 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1991-11-01 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology Regulation Bone Marrow Ige Mast Cells Proto Oncogenes Fos Mrna Pkc Activity Jun Proto Journal: Blood (Vol- 107, Issue- 9) |
| Content Type | Text |
| Resource Type | Article |
