Protein Kinase C Regulates Proliferation of Mast Cells and the Expression of the mRNAs offos andjun Proto-oncogenes During Activation by IgE-Ag or Calcium Ionophore A 23187

Content Provider	Semantic Scholar
Author	Razin, E.
Copyright Year	2003
Abstract	Short-term stimulation (up t o 16 hours) of interleukin-3 (IL-3)aependent mouse bone marrow-derived mast cells, Abelson transformed mouse liver-derived mast cells, or rat basophilic leukemia cells by either IgE-Ag or calcium ionophore A23187 resulted in inhibition of theirc proliferation as measured by 'H-thymidine incorporation and MTT (tetrazolium) assays, and in accumulation of the mRNAs of c-fos, c-jun, junB and slightly of jun0 proto-oncogenes. The involvement of protein kinase C (PKC) in these responses was investigated by using several approaches of enzyme activity regulation. Direct activation of the PKC was achieved by short-term exposure of the cells to the PKC-specific activator phorbol 12-myristate-13-acetate (PMA). Inhibition of PKC activity was obtained by either prolonged treatment of the cells with PMA or by exposure of the cells to the PKC inhibitors H-7 and staurosporine. The results showed the following: (1) Short-term exposure of mast cells to PMA was sufficient to induce inhibition of proliferation. (2) An increase in PKC activity was associated with a decrease in the proliferation of IgE-dinitrophenol (DNP) or calcium ionophore
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Alternate Webpage(s)	http://www.bloodjournal.org/content/bloodjournal/78/9/2354.full.pdf?sso-checked=1
Language	English
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Content Type	Text
Resource Type	Article