Loading...
Please wait, while we are loading the content...
Similar Documents
Direct radioimmune detection in human plasma of the association between factor VIII procoagulant protein and von Willebrand factor, and the interaction of von Willebrand factor-bound procoagulant VIII with platelets
| Content Provider | Scilit |
|---|---|
| Author | Moake, Jl Weinstein, Mj Troll, Jh Chute, Le Colannino, Nm |
| Copyright Year | 1983 |
| Description | The predominant procoagulant factor VIII (VIII:C) form in normal human plasma containing various combinations of anticoagulants and serine/cysteine protease inhibitors is a protein with mol wt 2.6 +/- 0.2 X 10(5). This protein can be detected by 125I-anti-VIII:C Fab binding and gel electrophoresis in the presence and absence of sodium dodecylsulfate (SDS) and is distinct from the subunit of factor VIII/von Willebrand factor (VIII:vWF) multimers. No larger VIII:C form is present in plasma from patients with severe congenital deficiencies of each of the coagulation factors, other than VIII:C. The mol wt approximately 2.6 X 10(5) VIII:C form is, therefore, likely to be the in vivo procoagulant form of VIII:C, rather than a partially proteolyzed, partially activated derivative of a larger precursor. About 60% of this procoagulant mol wt approximately 2.6 X 10(5) VIII:C form in plasma is present in noncovalent complexes with larger VIII:vWF multimers, which attach reversibly to platelet surfaces in the presence of ristocetin. This VIII:vWF-bound protein of mol wt approximately 2.6 X 10(5) may be the plasma procoagulant form of VIII:C which, after proteolytic activation, accelerates the IXa-mediated cleavage and activation of X postulated to occur on platelet surfaces. |
| Related Links | https://ashpublications.org/blood/article-pdf/61/6/1163/211939/1163.pdf |
| Ending Page | 1173 |
| Page Count | 11 |
| Starting Page | 1163 |
| DOI | 10.1182/blood.v61.6.1163.bloodjournal6161163 |
| Journal | Blood |
| Issue Number | 6 |
| Volume Number | 61 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1983-06-01 |
| Access Restriction | Open |
| Subject Keyword | Hematology Protein Sodium Plasma Bound Procoagulant Viii Willebrand Mol Wt Journal: Blood (Vol- 87, Issue- 6) |
| Content Type | Text |
| Resource Type | Article |
