NDLI: The complex multimeric composition of factor VIII/von Willebrand factor

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The complex multimeric composition of factor VIII/von Willebrand factor

Content Provider	Scilit
Author	Ruggeri, Zm Zimmerman, Ts
Copyright Year	1981
Description	We have analyzed the multimeric structure of factor VIII/von Willebrand factor in plasma by sodium dodecyl sulfate electrophoresis using gels of varying porosity and a discontinuous buffer system. Factor VIII/von Willebrand factor bands were identified by reaction with 125I-labeled affinity-purified antibody and subsequent autoradiography. In 1% agarose gels, normal plasma displayed a series of sharply defined oligomers. However, increasing the agarose concentration to 2.0% or utilizing mixtures of 0.8% agarose--1.75% acrylamide revealed two bands of lesser intensity interposed between the major bands. When the acrylamide concentration in the gels was increased to 2.5%, bands with a faster mobility than IgM and fibronectin were now evident. Type IIA von Willebrand's disease showed not only an absence of the larger multimers but also a relative increase in several of the newly identified bands as compared to type IIB, type I, and normal. These studies suggest that factor VII/von Willebrand factor in IIA von Willebrand's disease is structurally different from that in other forms of the disorder. They also indicate that the multimeric composition of factor VII/von Willebrand factor is more complex than can be explained by simple linear polymerization of a single protomer.
Related Links	https://ashpublications.org/blood/article-pdf/57/6/1140/208555/1140.pdf
Ending Page	1143
Page Count	4
Starting Page	1140
DOI	10.1182/blood.v57.6.1140.bloodjournal5761140
Journal	Blood
Issue Number	6
Volume Number	57
Language	English
Publisher	American Society of Hematology
Publisher Date	1981-06-01
Access Restriction	Open
Subject Keyword	Analytical Chemistry Antibody Gels Agarose Sodium Multimeric Structurally Viii/von Willebrand Factor Journal: Blood (Vol- 87, Issue- 6)
Content Type	Text
Resource Type	Article

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