NDLI: Donor-derived cells and human graft-versus-host disease of the skin

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Donor-derived cells and human graft-versus-host disease of the skin

Content Provider	Scilit
Author	Murata, Hideyuki Janin, Anne Leboeuf, Christophe Soulier, Jean Gluckman, Eliane Meignin, Veronique Socie, Gerard
Copyright Year	2006
Description	Graft-versus-host disease (GvHD)–induced apoptosis of the skin targets both epidermal keratinocytes and dermal endothelial cells. We studied the donor-versus-recipient origin of GvHD of these target cells in skin of 18 sex-mismatched hematopoietic stem-cell transplant (HSCT) recipients. Combining XY fluorescence in situ hybridization (FISH) and double immunostaining, and further 3D tissue Z-stack analysis, we found keratinocytes and endothelial cells of donor origin, but only in patients with GvHD. Using terminal dUTP nick-end labeling (TUNEL) assay on sister sections, we found a correlation between the numbers of chimeric and apoptotic epidermal and endothelial cells. Moreover, donor-derived cells were more numerous and preferentially distributed in the areas of severe GvHD damage in biopsies performed early in the course of GvHD, whereas they were less numerous and found in the whole epidermis in late biopsies. Because donor-derived cells were found at the site and at the time of maximum tissue damage, they could contribute to epidermal and microvessel repair.
Related Links	http://www.bloodjournal.org/content/109/6/2663.full.pdf
Ending Page	2665
Page Count	3
Starting Page	2663
DOI	10.1182/blood-2006-07-033902
Journal	Blood
Issue Number	6
Volume Number	109
Language	English
Publisher	American Society of Hematology
Publisher Date	2006-11-14
Access Restriction	Open
Subject Keyword	Research and Experimental Medicine Endothelial Cells Donor Derived Gvhd Apoptosis Hematopoietic Graft Recipients Biopsies Derived Cells Journal: Blood (Vol- 90, Issue- 6)
Content Type	Text
Resource Type	Article

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