Loading...
Please wait, while we are loading the content...
Similar Documents
Rescue of Cardiac Defects in Id Knockout Embryos by Injection of Embryonic Stem Cells
| Content Provider | Scilit |
|---|---|
| Author | Fraidenraich, Diego Stillwell, Elizabeth Romero, Elizabeth Wilkes, David Manova, Katia Basson, Craig T. Benezra, Robert |
| Copyright Year | 2004 |
| Description | We report that Id knockout mouse embryos display multiple cardiac defects, but mid-gestation lethality is rescued by the injection of 15 wild-type embryonic stem (ES) cells into mutant blastocysts. Myocardial markers altered in Id mutant cells are restored to normal throughout the chimeric myocardium. Intraperitoneal injection of ES cells into female mice before conception also partially rescues the cardiac phenotype with no incorporation of ES cells. Insulin-like growth factor 1, a long-range secreted factor, in combination with WNT5a, a locally secreted factor, likely account for complete reversion of the cardiac phenotype. Thus, ES cells have the potential to reverse congenital defects through Id-dependent local and long-range effects in a mammalian embryo. |
| Related Links | http://europepmc.org/articles/pmc1351017?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1351017/pdf https://science.sciencemag.org/content/sci/306/5694/247.full.pdf |
| Ending Page | 252 |
| Page Count | 6 |
| Starting Page | 247 |
| ISSN | 00368075 |
| e-ISSN | 10959203 |
| DOI | 10.1126/science.1102612 |
| Journal | Science |
| Issue Number | 5694 |
| Volume Number | 306 |
| Language | English |
| Publisher | American Association for the Advancement of Science (AAAS) |
| Publisher Date | 2004-10-08 |
| Access Restriction | Open |
| Subject Keyword | Journal: Science Cell Tissue Engineering Embryonic Stem Cells |
| Content Type | Text |
| Resource Type | Article |
| Subject | History and Philosophy of Science |
