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Induced Pluripotent Stem Cells Generated Without Viral Integration
| Content Provider | Scilit |
|---|---|
| Author | Stadtfeld, Matthias Nagaya, Masaki Utikal, Jochen Weir, Gordon Hochedlinger, Konrad |
| Copyright Year | 2008 |
| Description | Pluripotent stem cells have been generated from mouse and human somatic cells by viral expression of the transcription factors Oct4, Sox2, Klf4, and c-Myc. A major limitation of this technology is the use of potentially harmful genome-integrating viruses. We generated mouse induced pluripotent stem (iPS) cells from fibroblasts and liver cells by using nonintegrating adenoviruses transiently expressing Oct4, Sox2, Klf4, and c-Myc. These adenoviral iPS (adeno-iPS) cells show DNA demethylation characteristic of reprogrammed cells, express endogenous pluripotency genes, form teratomas, and contribute to multiple tissues, including the germ line, in chimeric mice. Our results provide strong evidence that insertional mutagenesis is not required for in vitro reprogramming. Adenoviral reprogramming may provide an improved method for generating and studying patient-specific stem cells and for comparing embryonic stem cells and iPS cells. |
| Related Links | http://europepmc.org/articles/pmc3987909?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3987909/pdf https://science.sciencemag.org/content/sci/322/5903/945.full.pdf |
| Ending Page | 949 |
| Page Count | 5 |
| Starting Page | 945 |
| ISSN | 00368075 |
| e-ISSN | 10959203 |
| DOI | 10.1126/science.1162494 |
| Journal | Science |
| Issue Number | 5903 |
| Volume Number | 322 |
| Language | English |
| Publisher | American Association for the Advancement of Science (AAAS) |
| Publisher Date | 2008-11-07 |
| Access Restriction | Open |
| Subject Keyword | Journal: Science Cell Tissue Engineering Cell Line Insertional Mutagenesis Pluripotent Stem Cells Transcription Factor Cell Differentiation Stem Cell Somatic Cells Embryonic Stem Cell |
| Content Type | Text |
| Resource Type | Article |
| Subject | History and Philosophy of Science |
