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Manipulation of Mouse Embryonic Stem Cells for Knockout Mouse Production
| Content Provider | Scilit |
|---|---|
| Author | Limaye, Advait Hall, Bradford Kulkarni, Ashok B. |
| Copyright Year | 2009 |
| Description | Journal: Current Protocols in Cell Biology The establishment of mouse embryonic stem (ES) cell lines has allowed for the gene?ration of the knockout mouse. ES cells that are genetically altered in culture can then be manipulated to derive a whole mouse containing the desired mutation. To successfully generate a knockout mouse, however, the ES cells must be carefully cultivated in a pluripotent state throughout the gene‐targeting experiment. This unit describes detailed step‐by‐step protocols, reagents, equipment, and strategies needed for the successful generation of gene knockout embryonic stem cells using homologous recombination technologies. Curr. Protoc. Cell Biol. 44:19.13.1‐19.13.24. © 2009 by John Wiley & Sons, Inc. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2759096/pdf |
| ISSN | 19342500 |
| e-ISSN | 19342616 |
| DOI | 10.1002/0471143030.cb1913s44 |
| Journal | Current Protocols in Cell Biology |
| Issue Number | 1 |
| Volume Number | 44 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2009-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Current Protocols in Cell Biology Cell Tissue Engineering Mouse Embryonic Stem Cells Homologous Recombination |
| Content Type | Text |
| Subject | Cell Biology Medicine |