NDLI: STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CIPROFLOXACIN AND DEXAMETHASONE IN BINARY COMBINATION

Content Provider	Scientific Electronic Library Online (SciELO)
Author	RAZZAQ, SYED NAEEM ASHFAQ, MUHAMMAD MARIAM, IRFANA KHAN, ISLAM ULLAH RAZZAQ, SYED SALEEM MUSTAFA, GHULAM
Abstract	ABSTRACT A simple and isocratic HPLC method with stability indicating nature was developed and then subsequently validated for simultaneous determination of ciprofloxacin and dexamethasone in pharmaceutical formulations, human serum and urine. Best chromatographic separations were attained within run time of 10 minutes using C8 as stationary phase and mixture of phosphate buffer and methanol (41:59 v/v) as mobile phase. The mobile phase was flowed at 1.5 mL min−1 with detection of both the analytes at 270 nm using photodiode array detector. Validation of the method was accomplished using specificity, linearity, accuracy, precision, robustness, LOD and LOQ. The method was found linear from 3-21 mg mL−1 for ciprofloxacin (r2 ≥ 0.999) and 1-7 mg mL−1 for dexamethasone (r2 ≥ 0.999). The %age recoveries of ciprofloxacin in spiked human urine and serum were ≥99% and ≥85% respectively, while for dexamethasone they were ≥97% in both matrices. The method proficiently separated the peaks of ciprofloxacin and dexamethasone from all types of interfering substances including degradation products/impurities with purity index ≥ 0.9998. The method thus was stability-indicating and can be employed for simultaneous analysis of ciprofloxacin and dexamethasone in complex matrices involving multiple components in the mixture.
Related Links	https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-97072017000303572&lng=en&nrm=iso https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000303572&lng=en&nrm=iso
Starting Page	3572
Ending Page	3577
Page Count	6
File Format	PDF
ISSN	07179707
DOI	10.4067/s0717-97072017000303572
Journal	Journal of the Chilean Chemical Society
Issue Number	3
Volume Number	62
Language	English
Publisher	Sociedad Chilena de Química
Publisher Date	2017-09-01
Access Restriction	Open
Subject Keyword	Liquid Chromatographic Ciprofloxacin Dexamethasone Degradation Products ICH Guidelines
Content Type	Text
Resource Type	Article
Subject	Chemistry

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